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PXD004645

PXD004645 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphoproteomic analyses of IL-2 signaling reveals integrated JAK-dependent and independent phosphorylation networks that drive cytotoxic T cell fate
DescriptionHigh-resolution mass spectrometry analysis of Interleukin 2 (IL-2) and Janus kinase (JAK) controlled protein phosphorylations in cytotoxic T lymphocytes (CTL) revealed JAKs coupled IL-2 receptors to diverse and complex serine/threonine kinase-substrate networks. These involved intricate, co-ordinated phosphorylation of transcription factors, chromatin regulators within the nuclear environment, cytosolic mRNA translational machinery, regulators of GTPases, vesicle trafficking proteins and the actin and microtubule cytoskeleton. We also identified an IL-2-JAK independent SRC family Tyr kinase controlled signaling network that regulates ~10% of the CTL phosphoproteome. One key signaling pathway in CTL is mediated by phosphatidylinositol (3,4,5)-trisphosphate (PIP3) and the serine/threonine kinase AKT. Strikingly, SRC family kinase dependent but JAK independent signaling controlled PIP3 levels and AKT activity in CTL. IL-2-JAK controlled signaling pathways thus coordinate with IL-2 independent networks of protein phosphorylation to program CTL fate.
HostingRepositoryPRIDE
AnnounceDate2016-08-25
AnnouncementXMLSubmission_2016-08-25_07:04:32.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSarah Ross
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue; monohydroxylated residue; acetylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-07-25 01:35:34ID requested
12016-08-25 07:04:33announced
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: IL-2, JAK1, JAK3,Lck/Fyn, SILAC, phosphoproteomics, lymphocytes.
Contact List
Doreen Ann Cantrell
contact affiliationDivision of Cell Signalling and Immunology, School of Life Sciences, University of Dundee, Dundee, UK
contact emaild.a.cantrell@dundee.ac.uk
lab head
Sarah Ross
contact affiliationUniversity of Dundee
contact emails.t.ross@dundee.ac.uk
dataset submitter
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Dataset FTP location
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