PXD004637

PXD004637 is an original dataset announced via ProteomeXchange.

Title	Label-free analysis of CD8+ T cell subset proteomes supports a progressive differentiation model of virus-specific T cells in humans
Description	Different pathogens trigger naïve T cells to express distinct sets of effector proteins. To better understand the molecular mechanisms that drive this functional specification, we used high resolution, label-free mass spectrometry to measure proteomic differences between the seven largest circulating human CD8+ T cell subsets. Unsupervised hierarchical clustering of the proteomes placed naïve and CD45RA-expressing effector-type T cells at the extremes of the spectrum with central-memory and other effector-memory stages located in between. Prominent differences between the subsets included expression of various granzymes, signaling proteins and molecules involved in metabolic regulation. Remarkably, whereas most of the proteomic changes between the subsets were gradual, a small proportion of proteins were regulated only in discrete subsets. The data obtained from this proteome analysis correspond best to a progressive differentiation model in which specific stable traits are gradually acquired during pathogen-specific development.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:31:25.559.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maartje van den Biggelaar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-21 07:20:57	ID requested
1	2017-05-02 01:18:52	announced
2	2019-02-12 08:10:16	announced	Updated publication reference for PubMed record(s): 28467900.
⏵ 3	2024-10-22 04:31:33	announced	2024-10-22: Updated project metadata.

10.1016/j.celrep.2017.04.014;

van Aalderen MC, van den Biggelaar M, Remmerswaal EBM, van Alphen FPJ, Meijer AB, Ten Berge IJM, van Lier RAW, T Cell Subset Proteomes Supports a Progressive Differentiation Model of Human-Virus-Specific T Cells. Cell Rep, 19(5):1068-1079(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Human, CD8+ T cells

Maartje van den Biggelaar
contact affiliation	Department of Plasma Proteins, Sanquin Research, Amsterdam, the Netherlands
contact email	m.vandenbiggelaar@sanquin.nl
lab head
Maartje van den Biggelaar
contact affiliation	Sanquin Research
contact email	m.vandenbiggelaar@sanquin.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD004637

PRIDE project URI

• PRIDE
  1. PXD004637
     1. Label: PRIDE project
     2. Name: Label-free analysis of CD8+ T cell subset proteomes supports a progressive differentiation model of virus-specific T cells in humans