PXD004635

PXD004635 is an original dataset announced via ProteomeXchange.

Title	Identification of novel Coronin 1A interacting proteins
Description	Severe combined immunodeficiency 8 (IMD8) is caused by mutations in the human Coronin 1A (Coro1A). The clinical presentation of IMD8 patients is characterized by recurrent bacterial infections, suggesting an important role of Coro1A in innate immunity. To analyze the molecular mechanism of Coro1A during neutrophil recruitment, we identified the Coro1A interactome by conducting co-immunoprecipitation (Co-IP) experiments using GFP NanoTrap technology and subsequent mass spectrometry (LC-MS/MS) using human neutrophil-like differentiated HL-60 (dHL-60) cells stably expressing Coro1A-EGFP (dHL-60-Coro1A-EGFP) cells.
HostingRepository	PRIDE
AnnounceDate	2017-06-29
AnnouncementXML	Submission_2017-06-29_01:36:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-21 07:04:11	ID requested
⏵ 1	2017-06-29 01:36:32	announced

Pick R, Begandt D, Stocker TJ, Salvermoser M, Thome S, B, ö, ttcher RT, Montanez E, Harrison U, Forn, é I, Khandoga AG, Coletti R, Weckbach LT, Brechtefeld D, Haas R, Imhof A, Massberg S, Sperandio M, Walzog B, Coronin 1A, a novel player in integrin biology, controls neutrophil trafficking in innate immunity. Blood, 130(7):847-858(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Coronin 1A, HL-60 cells, Co-immunoprecipitation, GFP nanotraps, LC-MS/MS, MaxQuant

Barbara Walzog
contact affiliation	Walter Brendel Centre of Experimental Medicine Department of Cardiovascular Physiology and Pathophysiology Ludwig-Maximilians-University of Munich Germany
contact email	walzog@lrz.uni-muenchen.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/06/PXD004635

PRIDE project URI

• PRIDE
  1. PXD004635
     1. Label: PRIDE project
     2. Name: Identification of novel Coronin 1A interacting proteins