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PXD004634

PXD004634 is an original dataset announced via ProteomeXchange.

Dataset Summary

  • Title: Deep Biosphere - Beatrix 326 BH2 (270712)
  • Description: This project aims to investigate the metabolic pathways expressed by the active microbial community occurring at the deep continental subsurface. Subsurface chemoLithoautotrophic Microbial Ecosystems (SLiMEs) under oligotrophic conditions are supported by H2; however, the overall ecological trophic structures of these communities are poorly understood. Some deep, fluid-filled fractures in the Witwatersrand Basin, South Africa appear to support inverted trophic pyramids wherein methanogens contributing <5% of the total DNA apparently produce CH4 that supports the rest of the community. Here we show the active metabolic relationships of one such trophic structure by combining metatranscriptomic assemblies, metaproteomic and stable isotopic data, and thermodynamic modeling. Four autotrophic β-proteobacteria genera that are capable of oxidizing sulfur by denitrification dominate. They co-occur with sulfate reducers, anaerobic methane oxidizers and methanogens, which each comprises <5% of the total community. Defining trophic levels of microbial chemolithoautotrophs by the number of transfers from the initial abiotic H2-driven CO2 fixation, we propose a top-down cascade influence of the metabolic consumers that enhances the fitness of the metabolic producers to explain the inverted biomass pyramid of a multitrophic SLiME. Symbiotic partnerships are pivotal in the deep biosphere on and potentially beyond the Earth.
  • HostingRepository: PRIDE
  • AnnounceDate: 2018-10-29
  • AnnouncementXML: Submission_2018-10-29_07:13:34.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original dataset
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Maggie Lau
  • SpeciesList: scientific name: Sulfuritalea; NCBI TaxID: 1054211;
  • ModificationList: monohydroxylated residue; iodoacetamide derivatized residue
  • Instrument: LTQ Orbitrap Elite

Dataset History

RevisionDatetimeStatusChangeLog Entry
02016-07-21 05:53:00ID requested
12018-10-29 07:13:35announced

Publication List

  1. Lau MCY, Harris RL, Oh Y, Yi MJ, Behmard A, Onstott TC, Taxonomic and Functional Compositions Impacted by the Quality of Metatranscriptomic Assemblies. Front Microbiol, 9():1235(2018) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: deep subsurface ecosystem, native microbial community, crustal fluid, ecological trophic structure, sample-derived metatranscripome database

Contact List

    Maggie Lau
    • contact affiliation: Onstott Lab, Department of Geosciences, Princeton University
    • contact email: maglau@princeton.edu
    • lab head:
    Maggie Lau
    • contact affiliation: Princeton University
    • contact email: maglau@princeton.edu
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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