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PXD004634

PXD004634 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDeep Biosphere - Beatrix 326 BH2 (270712)
DescriptionThis project aims to investigate the metabolic pathways expressed by the active microbial community occurring at the deep continental subsurface. Subsurface chemoLithoautotrophic Microbial Ecosystems (SLiMEs) under oligotrophic conditions are supported by H2; however, the overall ecological trophic structures of these communities are poorly understood. Some deep, fluid-filled fractures in the Witwatersrand Basin, South Africa appear to support inverted trophic pyramids wherein methanogens contributing <5% of the total DNA apparently produce CH4 that supports the rest of the community. Here we show the active metabolic relationships of one such trophic structure by combining metatranscriptomic assemblies, metaproteomic and stable isotopic data, and thermodynamic modeling. Four autotrophic β-proteobacteria genera that are capable of oxidizing sulfur by denitrification dominate. They co-occur with sulfate reducers, anaerobic methane oxidizers and methanogens, which each comprises <5% of the total community. Defining trophic levels of microbial chemolithoautotrophs by the number of transfers from the initial abiotic H2-driven CO2 fixation, we propose a top-down cascade influence of the metabolic consumers that enhances the fitness of the metabolic producers to explain the inverted biomass pyramid of a multitrophic SLiME. Symbiotic partnerships are pivotal in the deep biosphere on and potentially beyond the Earth.
HostingRepositoryPRIDE
AnnounceDate2018-10-29
AnnouncementXMLSubmission_2018-10-29_07:13:34.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMaggie Lau
SpeciesList scientific name: Sulfuritalea; NCBI TaxID: 1054211;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-07-21 05:53:00ID requested
12018-10-29 07:13:35announced
Publication List
Lau MCY, Harris RL, Oh Y, Yi MJ, Behmard A, Onstott TC, Taxonomic and Functional Compositions Impacted by the Quality of Metatranscriptomic Assemblies. Front Microbiol, 9():1235(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: deep subsurface ecosystem, native microbial community, crustal fluid, ecological trophic structure, sample-derived metatranscripome database
Contact List
Maggie Lau
contact affiliationOnstott Lab, Department of Geosciences, Princeton University
contact emailmaglau@princeton.edu
lab head
Maggie Lau
contact affiliationPrinceton University
contact emailmaglau@princeton.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
Repository Record List
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