PXD004630

PXD004630 is an original dataset announced via ProteomeXchange.

Title	ON-TISSUE DIRECT MONITORING OF GLOBAL HYDROGEN/DEUTERIUM EXCHANGE BY MALDI MASS SPECTROMETRY: TDXMS
Description	Hydrogen/deuterium exchange mass spectrometric methods for protein structural analysis are conventionally performed in solution. We present Tissue Deuterium Exchange Mass Spectrometry (TDXMS), a method to directly monitor deuterium uptake on tissue, as a means to better approximate the deuterium exchange behavior of proteins in their native microenvironment. Using this method, a difference in the deuterium uptake behavior was observed when the same proteins were monitored in solution and on tissue. The higher maximum deuterium uptake at equilibrium for all proteins analyzed in solution suggests a more open conformation in the absence of interacting partners normally observed on tissue. We also demonstrate a difference in the deuterium uptake behavior of a few proteins across different morphological regions of the same tissue section. Modifications of the total number of hydrogens exchanged, as well as the kinetics of exchange, were both observed. These results provide information on the implication of protein interactions with partners as well as on the conformational changes related to these interactions, and illustrate the importance of examining protein deuterium exchange behavior in the presence of its specific microenvironment directly at the level of tissues.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:31:43.251.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jusal Quanico
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-21 03:28:28	ID requested
1	2016-08-15 02:04:49	announced
⏵ 2	2024-10-22 04:31:44	announced	2024-10-22: Updated project metadata.

Quanico J, Franck J, Salzet M, Fournier I, On-tissue Direct Monitoring of Global Hydrogen/Deuterium Exchange by MALDI Mass Spectrometry: Tissue Deuterium Exchange Mass Spectrometry (TDXMS). Mol Cell Proteomics, 15(10):3321-3330(2016) [pubmed]

curator keyword: Technical

submitter keyword: tissue, mass spectrometry,In situ analysis, H/D exchange

Michel Salzet
contact affiliation	Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM) INSERM U1192- Université Lille 1 Bât SN3, 1er étage, porte 107 F-59655 Villeneuve d’Ascq Cedex France
contact email	michel.salzet@univ-lille1.fr
lab head
Jusal Quanico
contact affiliation	PRISM- University of Lille 1
contact email	jusal.quanico@univ-lille1.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004630

PRIDE project URI

• PRIDE
  1. PXD004630
     1. Label: PRIDE project
     2. Name: ON-TISSUE DIRECT MONITORING OF GLOBAL HYDROGEN/DEUTERIUM EXCHANGE BY MALDI MASS SPECTROMETRY: TDXMS