PXD004630 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | ON-TISSUE DIRECT MONITORING OF GLOBAL HYDROGEN/DEUTERIUM EXCHANGE BY MALDI MASS SPECTROMETRY: TDXMS |
Description | Hydrogen/deuterium exchange mass spectrometric methods for protein structural analysis are conventionally performed in solution. We present Tissue Deuterium Exchange Mass Spectrometry (TDXMS), a method to directly monitor deuterium uptake on tissue, as a means to better approximate the deuterium exchange behavior of proteins in their native microenvironment. Using this method, a difference in the deuterium uptake behavior was observed when the same proteins were monitored in solution and on tissue. The higher maximum deuterium uptake at equilibrium for all proteins analyzed in solution suggests a more open conformation in the absence of interacting partners normally observed on tissue. We also demonstrate a difference in the deuterium uptake behavior of a few proteins across different morphological regions of the same tissue section. Modifications of the total number of hydrogens exchanged, as well as the kinetics of exchange, were both observed. These results provide information on the implication of protein interactions with partners as well as on the conformational changes related to these interactions, and illustrate the importance of examining protein deuterium exchange behavior in the presence of its specific microenvironment directly at the level of tissues. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:31:43.251.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jusal Quanico |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-07-21 03:28:28 | ID requested | |
1 | 2016-08-15 02:04:49 | announced | |
⏵ 2 | 2024-10-22 04:31:44 | announced | 2024-10-22: Updated project metadata. |
Publication List
Quanico J, Franck J, Salzet M, Fournier I, On-tissue Direct Monitoring of Global Hydrogen/Deuterium Exchange by MALDI Mass Spectrometry: Tissue Deuterium Exchange Mass Spectrometry (TDXMS). Mol Cell Proteomics, 15(10):3321-3330(2016) [pubmed] |
Keyword List
curator keyword: Technical |
submitter keyword: tissue, mass spectrometry,In situ analysis, H/D exchange |
Contact List
Michel Salzet |
contact affiliation | Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM) INSERM U1192- Université Lille 1 Bât SN3, 1er étage, porte 107 F-59655 Villeneuve d’Ascq Cedex France |
contact email | michel.salzet@univ-lille1.fr |
lab head | |
Jusal Quanico |
contact affiliation | PRISM- University of Lille 1 |
contact email | jusal.quanico@univ-lille1.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD004630 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004630
- Label: PRIDE project
- Name: ON-TISSUE DIRECT MONITORING OF GLOBAL HYDROGEN/DEUTERIUM EXCHANGE BY MALDI MASS SPECTROMETRY: TDXMS