PXD004622

PXD004622 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Human Fibroblast cell_Tig120slc-P8
Description	Proteome analyses of human fibroblast cell line (Tig120slc-P8) were carried out using a two-meter monolithic silica C18 capillary columns without prefractionation. Tryptic peptides from 4 μg cell lysates were directly injected onto the 100 μm i.d. monolithic silica-C18 column and an 8-h gradient was applied at 500 nL/min. Triplicate measurements were conducted and the merged results were searched against UniProt DB to identify peptides and proteins. The obtained results were filtered at 1% FDR thresholds by searching against the randomized decoy DB both at peptide and protein levels. This dataset with the PX complete submission format will be further used to generate a customized DB in this jPOST project.
HostingRepository	jPOST
AnnounceDate	2016-07-21
AnnouncementXML	Submission_2022-09-18_03:36:29.034.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD004622
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michio Funahashi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-20 19:49:27	ID requested
1	2016-07-20 20:48:27	announced
2	2016-11-17 18:11:18	announced	Updated description.
3	2017-03-30 23:31:54	announced	Updated Repository support.
4	2017-03-31 00:05:44	announced	Updated DigitalObjectIdentifier.
5	2018-09-18 22:41:57	announced	Updated CV, FTP location and Modification list.
6	2021-02-04 17:27:27	announced	2021-02-04: Updated FTP location.
⏵ 7	2022-09-18 03:36:29	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: shotgun proteomics, monolithic silica column, iPS cell, one-shot proteomics

submitter keyword: shotgun proteomics, monolithic silica column, iPS cell, one-shot proteomics

Contact List

Yasushi Ishihama
lab head
Michio Funahashi
contact affiliation	Kyoto university
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000089/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000089/