PXD004613

PXD004613 is an original dataset announced via ProteomeXchange.

Title	Repo-Man/PP1 regulates heterochromatin formation in interphase
Description	GST:Repo-Man(C-terminus) or GST:alone were expressed as in Vagnarelli et al. 2011. Before elution, beads were first incubated with nucleosomes extracted from HeLa. HeLa cells were lysed and nucleosomes were digested with MNase (20min, 37C). Nucleosomes were firstly incubated with GST alone for 2hrs. This cleared lysate was then incubated with GST:Repo-Man and GST alone. Beads were then eluted and mix with 3x Laemli Buffer. Histone bands were sent to Mass Spec.
HostingRepository	PRIDE
AnnounceDate	2016-12-07
AnnouncementXML	Submission_2017-06-12_07:29:49.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paola Vagnarelli
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-20 06:08:43	ID requested
1	2016-12-07 07:44:40	announced
⏵ 2	2017-06-12 07:29:50	announced	Updated publication reference for PubMed record(s): 28091603.

de Castro IJ, Budzak J, Di Giacinto ML, Ligammari L, Gokhan E, Spanos C, Moralli D, Richardson C, de Las Heras JI, Salatino S, Schirmer EC, Ullman KS, Bickmore WA, Green C, Rappsilber J, Lamble S, Goldberg MW, Vinciotti V, Vagnarelli P, Repo-Man/PP1 regulates heterochromatin formation in interphase. Nat Commun, 8():14048(2017) [pubmed]

curator keyword: Biological

submitter keyword: Human, HeLa, Repo-Man, Shotgun proteomics heretochromatin

Paola Vagnarelli
contact affiliation	Brunel University London
contact email	Paola.Vagnarelli@brunel.ac.uk
lab head
Paola Vagnarelli
contact affiliation	Brunel University London
contact email	Paola.Vagnarelli@brunel.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD004613

PRIDE project URI

• PRIDE
  1. PXD004613
     1. Label: PRIDE project
     2. Name: Repo-Man/PP1 regulates heterochromatin formation in interphase