PXD004611

PXD004611 is an original dataset announced via ProteomeXchange.

Title	Somatic embryogenesis of Pinus nigra
Description	There are several issues complicating somatic embryogenesis in conifers, for instance the low initiation frequency, the weak maturation capacity, and the loss of an embryogenic potential after a prolonged cultivation. The aim of our study was to clarify molecular details of the this process in the tree Pinus nigra Arn. We performed comparative proteomic analysis based on the two-dimensional gel electrophoresis in 2 genotypes coded 362 and 366 of: 1) proliferating embryogenic tissues (E) initiated from immature zygotic embryos, 2) non-embryogenic calli (NEC) started from cotyledons of somatic seedlings, 3) embryogenic tissues that lost the maturation capacity (E-L). Pine tissues showed distinct morphologic features: a) E362 and E366 were characterized by the presence of early bipolar structures capable of maturation and plantlet regeneration; b) NEC362 and NEC366 were composed of round shaped cells without any organisation; c) for E-L362 and E-L366 the long-term culture of initially embryogenic tissues resulted in the disorganisation of early bipolar structures. We found 24 differentially accumulated protein spots common for both genotypes after comparison of E versus NEC.
HostingRepository	PRIDE
AnnounceDate	2017-09-06
AnnouncementXML	Submission_2017-09-06_07:51:22.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD004611
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Maksym Danchenko
SpeciesList	scientific name: Pinus nigra; NCBI TaxID: 58042;
ModificationList	monomethylated residue; phosphorylated residue; deaminated residue; monohydroxylated residue; acetylated residue; monosodium salt; iodoacetamide derivatized residue; deamidated residue
Instrument	ultrafleXtreme

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-20 06:06:28	ID requested
⏵ 1	2017-09-06 07:51:23	announced

Klubicov, á K, Uv, á, ckov, á L, Danchenko M, Nemecek P, Skult, é, ty L, Salaj J, Salaj T, Insights into the early stage of Pinus nigra Arn. somatic embryogenesis using discovery proteomics. J Proteomics, 169():99-111(2017) [pubmed]

curator keyword: Biological

submitter keyword: Cell wall biosynthesis

Conifers

Embryogenic tissue

Loss of maturation capacity

Non-embryogenic callus

2-D gel electrophoresis

Katarína Klubicová
contact affiliation	Institute of Plant Genetics and Biotechnology, Plant Science and Biodiversity Center SAS
contact email	katarina.klubicova@savba.sk
lab head
Maksym Danchenko
contact affiliation	Institute of Virology, Biomedical Research Center SAS
contact email	maxymdan@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD004611

PRIDE project URI

• PRIDE
  1. PXD004611
     1. Label: PRIDE project
     2. Name: Somatic embryogenesis of Pinus nigra