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PXD004608

PXD004608 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIntegrated cellular and plasma proteomics reveals common and systemic signatures in contrasting B-cell cancers
DescriptionApproximately 800,000 leukaemia and lymphoma cases are diagnosed worldwide each year. Burkitt’s lymphoma and chronic lymphocytic leukaemia represent contrasting examples of B-cell cancers, modelled by Eμ-myc and Eμ-TCL1 mice, respectively. To better understand B-cell cancers and systemic effects of oncogenesis, tumours and plasma from these models were profiled by mass spectrometry proteomics. 8270 cellular and 2095 plasma proteins were fully quantitated by isobaric labelling, of which 695 and 279 demonstrated overabundance coinciding in both tumour models, respectively. Co-occurring upregulated cellular tumour processes included ribosome biogenesis, translation, cell cycle promotion and chromosome segregation. Tumour plasma overabundance highlighted immunity, inflammation, microenvironment interactions, a prolific tumour lysis signature and putative biomarkers of early-stage cancer. Integrative evaluation of tumours and plasma provided systemic insight not captured in isolation. Overall, these findings provide a detailed characterisation of systemic oncogenesis in two contrasting B-cell tumour models, identifying extensive common profiles in both tumour cells and plasma.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:31:26.681.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHarvey Johnston
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; methylthiolated residue; phosphorylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-07-20 01:37:11ID requested
12017-01-11 05:11:35announced
22017-10-24 04:42:55announcedUpdated project metadata.
32024-10-22 04:31:30announced2024-10-22: Updated project metadata.
Publication List
10.1074/mcp.M116.063511;
Johnston HE, Carter MJ, Cox KL, Dunscombe M, Manousopoulou A, Townsend PA, Garbis SD, Cragg MS, Integrated Cellular and Plasma Proteomics of Contrasting B-cell Cancers Reveals Common, Unique and Systemic Signatures. Mol Cell Proteomics, 16(3):386-406(2017) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Eµ-myc, lymphoma, B-cell, oncogenesis, proteomics, Eµ-TCL1, plasma, leukaemia
Contact List
Spiro Dennis Garbis
contact affiliationClinical and Experimental Sciences Unit, Faculty of Medicine, University of Southampton, Southampton SO16 6YD, UK Centre for Proteomic Research, Institute for Life Sciences, University of Southampton, Highfield Campus, Southampton, SO17 1BJ, UK
contact emails.d.garbis@soton.ac.uk
lab head
Harvey Johnston
contact affiliationBabraham Institute, Cambridge, UK
contact emailh.johnston@soton.ac.uk
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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