PXD004607

PXD004607 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Quantitative mass spectrometry analysis of intestinal organoids
Description	Organoids have the potential to bridge 3D cell culture to tissue physiology by providing a model resembling in vivo organs. We propose here a SILAC method to measure protein expression changes in intestinal organoids under different experimental conditions. With the combined use of quantitative mass spectrometry, SILAC and organoid culture, we validated the approach and showed that large-scale proteome variations can be measured in an “organ-like” system under variable conditions.
HostingRepository	PRIDE
AnnounceDate	2017-09-05
AnnouncementXML	Submission_2017-09-05_08:04:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francois-Michel Boisvert
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	6x(13)C labeled residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-20 01:30:14	ID requested
⏵ 1	2017-09-05 08:04:44	announced

Publication List

Gonneaud A, Jones C, Turgeon N, L, é, vesque D, Asselin C, Boudreau F, Boisvert FM, A SILAC-Based Method for Quantitative Proteomic Analysis of Intestinal Organoids. Sci Rep, 6():38195(2016) [pubmed]

Gonneaud A, Jones C, Turgeon N, L, é, vesque D, Asselin C, Boudreau F, Boisvert FM, A SILAC-Based Method for Quantitative Proteomic Analysis of Intestinal Organoids. Sci Rep, 6():38195(2016) [pubmed]

Keyword List

curator keyword: Biological, Biomedical
submitter keyword: Organoids, quantitative proteomics, SILAC, intestine

curator keyword: Biological, Biomedical

submitter keyword: Organoids, quantitative proteomics, SILAC, intestine

Contact List

Francois-Michel Boisvert
contact affiliation	Department of Anatomy and Cell Biology, Faculty of medecine and Health Sciences, Université de Sherbrooke
contact email	fm.boisvert@usherbrooke.ca
lab head
Francois-Michel Boisvert
contact affiliation	Université de Sherbrooke
contact email	fm.boisvert@usherbrooke.ca
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD004607
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD004607

PRIDE project URI

Repository Record List

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