PXD004606

PXD004606 is an original dataset announced via ProteomeXchange.

Title	Endothelial Cells from GvsHD Mouse
Description	During inflammation immune cells can induce endothelial activation and angiogenesis by cytokines and other mediators1,2. The inhibition of inflammation-associated angiogenesis ameliorates inflammatory diseases by reducing the recruitment of tissue infiltrating leukocytes3-5. However, there is limited evidence on initial mechanisms of both processes. Here we show that angiogenesis precedes leukocyte infiltration during graft-versus-host disease (GVHD) and experimental colitis. A key feature of GVHD is the incompletely understood organ tropism to skin, liver and the intestines. We found that angiogenesis initiates GVHD in target organs whereas in non-target organs no angiogenesis and no subsequent inflammation occur, suggesting a previously unrecognized role of the endothelium in GVHD organ tropism. The initiation phase of angiogenesis was not associated to classical endothelial cell (EC) activation signs, such as Vegfa/VEGFR1+2 upregulation or increased adhesion molecule expression. In gene array- and proteomic analyses, we found significant metabolic and cytoskeleton changes in ECs leading to profoundly higher deformation in real-time deformability cytometry6. Our results demonstrate that metabolic changes trigger enhanced migratory and proliferating potential of ECs during the initiation of angiogenesis in GVHD target organs. Our study adds evidence to the hypothesis that angiogenesis can initiate inflammation and provides novel insight in pathophysiology and tropism of GVHD.
HostingRepository	PRIDE
AnnounceDate	2017-01-31
AnnouncementXML	Submission_2017-01-31_02:09:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Perez Hernandez
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-19 23:24:25	ID requested
⏵ 1	2017-01-31 02:09:50	announced

Riesner K, Shi Y, Jacobi A, Kr, ä, ter M, Kalupa M, McGearey A, Mertlitz S, Cordes S, Schrezenmeier JF, Mengwasser J, Westphal S, Perez-Hernandez D, Schmitt C, Dittmar G, Guck J, Penack O, Initiation of acute graft-versus-host disease by angiogenesis. Blood, 129(14):2021-2032(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Endothelial cells, mouse, DML, GvsHD

Daniel Perez Hernandez
contact affiliation	Max-Delbrück-Centrum Berlin
contact email	daniel.perezhernandez@mdc-berlin.de
lab head
Daniel Perez Hernandez
contact affiliation	Max Delbruck Center
contact email	daniel.perezhernandez@mdc-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD004606

PRIDE project URI

• PRIDE
  1. PXD004606
     1. Label: PRIDE project
     2. Name: Endothelial Cells from GvsHD Mouse