PXD004596 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Dissecting the secretory pathway with electrophoresis reveals intra-Golgi trafficking pathways and enables compilation of sub-Golgi and sub-ER proteomes. |
Description | The Golgi is the hub of the eukaryotic secretory pathway, trafficking proteins and lipids, as well as synthesizing complex sugars. Different biosynthetic reactions are associated with different compartments of its complex architecture. Although pre- and post-Golgi trafficking has been much studied, comparatively little is known about intra-Golgi organization. In this study, secretory vesicles and organelles were separated along an electrophoretic gradient at sub-Golgi resolution, presenting snapshots of the changing relative abundance of hundreds of resident and cargo proteins and glycans in transit through the ER, Golgi compartments and post-Golgi compartments. Furthermore, grouped features in migration profiles reveal the dominant intra-Golgi protein trafficking pathways, showing separate routes for cargo and different groups of resident proteins. As few structural characteristics of proteins or sequence motifs have been associated with specific regions of the Golgi stack, we also carried out a comparative analysis of the transmembrane regions of resident proteins associated with the main migratory profiles identifying the presence of charge amino acids adjacent to the transmembrane helix, exoplasmic Ser and Thr content and helix composition as likely contributors to protein sorting mechanisms. |
HostingRepository | PRIDE |
AnnounceDate | 2019-09-11 |
AnnouncementXML | Submission_2019-09-11_09:06:48.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD004596 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Harriet Parsons |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 5600; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-07-19 02:07:20 | ID requested | |
⏵ 1 | 2019-09-11 09:06:50 | announced | |
Publication List
Parsons HT, Stevens TJ, McFarlane HE, Vidal-Melgosa S, Griss J, Lawrence N, Butler R, Sousa MML, Salemi M, Willats WGT, Petzold CJ, Heazlewood JL, Lilley KS, Reveals Underlying Properties of Cisternal Localization. Plant Cell, 31(9):2010-2034(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Golgi, cisternae, endoplasmic reticulum, secretory pathway, trafficking, Arabidopsis, proteomics, trans-membrane span, polysaccharide, immunogold, free flow electrophoresis |
Contact List
Kathryn s. Lilley |
contact affiliation | Department of Biochemistry University of Cambridge Cambridge UK |
contact email | ksl23@cam.ac.uk |
lab head | |
Harriet Parsons |
contact affiliation | Cambridge University |
contact email | tempeparsons@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004596
- Label: PRIDE project
- Name: Dissecting the secretory pathway with electrophoresis reveals intra-Golgi trafficking pathways and enables compilation of sub-Golgi and sub-ER proteomes.