PXD004592

PXD004592 is an original dataset announced via ProteomeXchange.

Title	A metaproteomic analysis of the response of a freshwater microbial community under nutrient enrichment
Description	Eutrophication can lead to an uncontrollable increase in algal biomass, which has repercussions for the entire microbial and pelagic community. Studies have shown how nutrient enrichment affects microbial species succession, however details regarding the impact on community functionality are rare. Here, we applied a metaproteomic approach to investigate the functional changes to algal and bacterial communities, over time, in oligotrophic and eutrophic conditions, in freshwater microcosms. Samples were taken early during algal and cyanobacterial dominance and later under bacterial dominance. 1048 proteins, from the two treatments and two timepoints, were identified and quantified by their exponentially modified protein abundance index. In oligotrophic conditions, Bacteroidetes express extracellular hydrolases and Ton-B dependent receptors to degrade and transport high molecular weight compounds captured while attached to the phycosphere. Alpha- and Beta-proteobacteria were found to capture different substrates from algal exudate (carbohydrates and amino acids, respectively) suggesting resource partitioning to avoid direct competition. In eutrophic conditions, environmental adaptation proteins from cyanobacteria suggested better resilience compared to algae in a low carbon nutrient enriched environment. This study provides insight into differences in functional microbial processes between oligo- and eutrophic conditions at different timepoints and highlights how primary producers control bacterial resources in freshwater environments.
HostingRepository	PRIDE
AnnounceDate	2016-08-01
AnnouncementXML	Submission_2017-06-12_07:30:21.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD004592
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Russo
SpeciesList	scientific name: mixed sample; NCBI TaxID: 1427524;
ModificationList	Methylthio; Oxidation
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-18 03:34:19	ID requested
1	2016-08-01 09:48:15	announced
⏵ 2	2017-06-12 07:30:22	announced	Updated publication reference for PubMed record(s): 27536273.

Russo DA, Couto N, Beckerman AP, Pandhal J, A Metaproteomic Analysis of the Response of a Freshwater Microbial Community under Nutrient Enrichment. Front Microbiol, 7():1172(2016) [pubmed]

curator keyword: Metaproteomics

submitter keyword: oligotrophic, eutrophic, metaproteomics, microbial loop, freshwater, LC-MSMS

Jagroop Pandhal
contact affiliation	University of Sheffield, Faculty of Engineering, Department of Chemical and Biological Engineering, UK
contact email	j.pandhal@sheffield.ac.uk
lab head
David Russo
contact affiliation	University of Sheffield
contact email	davidarusso@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004592
     1. Label: PRIDE project
     2. Name: A metaproteomic analysis of the response of a freshwater microbial community under nutrient enrichment