PXD004588

PXD004588 is an original dataset announced via ProteomeXchange.

Title	ZIKV infection of human neurospheres
Description	Zika virus (ZIKV) has been associated with microcephaly and other brain abnormalities; however, the molecular consequences of ZIKV to human brain development are still not fully understood. Here we describe alterations in human neurospheres derived from induced pluripotent stem (iPS) cells infected with the strain of Zika virus that is circulating in Brazil. Combining proteomics and mRNA transcriptional profiling, over 500 proteins and genes associated with the Brazilian ZIKV infection were found to be differentially expressed. These genes and proteins provide an interactome map, which indicates that ZIKV controls the expression of RNA processing bodies, miRNA biogenesis and splicing factors required for self-replication. It also suggests that impairments in the molecular pathways underpinning cell cycle and neuronal differentiation are caused by ZIKV. These results point to biological mechanisms implicated in brain malformations, which are important to further the understanding of ZIKV infection and can be exploited as therapeutic potential targets to mitigate it.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_03:58:28.397.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Caroline Teles
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-18 01:54:58	ID requested
1	2018-10-26 15:27:39	announced
⏵ 2	2024-10-22 03:58:29	announced	2024-10-22: Updated project metadata.

10.1038/srep40780;

Garcez PP, Nascimento JM, de Vasconcelos JM, Madeiro da Costa R, Delvecchio R, Trindade P, Loiola EC, Higa LM, Cassoli JS, Vit, ó, ria G, Sequeira PC, Sochacki J, Aguiar RS, Fuzii HT, de Filippis AM, da Silva Gon, ç, alves Vianez J, ú, nior JL, Tanuri A, Martins-de-Souza D, Rehen SK, Zika virus disrupts molecular fingerprinting of human neurospheres. Sci Rep, 7():40780(2017) [pubmed]

submitter keyword: microcephaly, iPSC,human, zika

Daniel Martins-de-Souza
contact affiliation	Lab of Neuroproteomics, Institute of Biology, Department of Biochemistry and Tissue Biology, University of Campinas (UNICAMP), Campinas, Brazil ( lab head )
contact email	dams90@gmail.com
lab head
Caroline Teles
contact affiliation	University of Campinas
contact email	carolbr.teles@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD004588

PRIDE project URI

• PRIDE
  1. PXD004588
     1. Label: PRIDE project
     2. Name: ZIKV infection of human neurospheres