PXD004567

PXD004567 is an original dataset announced via ProteomeXchange.

Title	High-throughput Proteomics Reveals the Unicellular Roots of Animal Phosphosignaling and Cell Differentiation
Description	Cell-specific regulation of protein levels and activity is essential for the distribution of functions among multiple cell types in animals. The finding that many genes involved in these regulatory processes have a premetazoan origin raises the intriguing possibility that the mechanisms required for spatially regulated cell differentiation evolved prior to the appearance of animals. Here, we use high-throughput proteomics in Capsaspora owczarzaki, a close unicellular relative of animals, to characterize the dynamic proteome and phosphoproteome profiles of three temporally distinct cell types in this premetazoan species. We show that life-cycle transitions are linked to extensive proteome and phosphoproteome remodeling and that they affect key genes involved in animal multicellularity, such as transcription factors and tyrosine kinases. The observation of shared features between Capsaspora and metazoans indicates that elaborate and conserved phosphosignaling and proteome regulation supported temporal cell-type differentiation in the unicellular ancestor of animals.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:31:46.415.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eduard Sabidó
SpeciesList	scientific name: Capsaspora owczarzaki; NCBI TaxID: 192875;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-13 01:25:42	ID requested
1	2016-11-30 03:16:31	announced
2	2017-10-24 05:52:10	announced	Updated project metadata.
⏵ 3	2024-10-22 04:31:52	announced	2024-10-22: Updated project metadata.

10.1016/j.devcel.2016.09.019;

Seb, é, -Pedr, ó, s A, Pe, ñ, a MI, Capella-Guti, é, rrez S, Ant, ó M, Gabald, ó, n T, Ruiz-Trillo I, Sabid, ó E, High-Throughput Proteomics Reveals the Unicellular Roots of Animal Phosphosignaling and Cell Differentiation. Dev Cell, 39(2):186-197(2016) [pubmed]

curator keyword: Biological

submitter keyword: metazoa,capsaspora

Eduard Sabidó
contact affiliation	Proteomics Unit, Centre de Regulació Genòmica, Dr. Aiguader 88, 08003 Barcelona
contact email	eduard.sabido@crg.cat
lab head
Eduard Sabidó
contact affiliation	Centre de Regulació Genòmica
contact email	eduard.sabido@crg.cat
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/11/PXD004567

PRIDE project URI

• PRIDE
  1. PXD004567
     1. Label: PRIDE project
     2. Name: High-throughput Proteomics Reveals the Unicellular Roots of Animal Phosphosignaling and Cell Differentiation