PXD004566

PXD004566 is an original dataset announced via ProteomeXchange.

Title	Membrane-bound electron transport systems of an anammox bacterium: a complexome analysis
Description	Electron transport, or oxidative phosphorylation, is one of the hallmarks of life. To this end, prokaryotes evolved a vast variety of protein complexes, only a small part of which have been discovered and studied. These protein complexes allow them to occupy virtually every ecological niche on Earth. Here, we applied the advanced method of proteomics-based complexome profiling to get a better understanding of the electron transport systems of the anaerobic ammonium-oxidizing (anammox) bacteria, the N2-producing key players of the global nitrogen cycle. By this method nearly all respiratory complexes that were previously predicted from genome analysis to be involved in energy and cell carbon fixation were validated. More importantly, new and unexpected ones were discovered. We believe that complexome profiling in concert with (meta)genomics offers great opportunities to expand our knowledge on bacterial respiratory processes at a rapid and massive pace, in particular in new and thus far poorly investigated non-model and environmentally-relevant species.
HostingRepository	PRIDE
AnnounceDate	2019-10-29
AnnouncementXML	Submission_2019-10-29_09:51:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hans Wessels
SpeciesList	scientific name: Candidatus Kuenenia stuttgartiensis; NCBI TaxID: 174633;
ModificationList	carbamoylated residue; monohydroxylated residue; acetylated residue
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-13 01:20:43	ID requested
⏵ 1	2019-10-29 09:51:19	announced

de Almeida NM, Wessels HJ, de Graaf RM, Ferousi C, Jetten MS, Keltjens JT, Kartal B, Membrane-bound electron transport systems of an anammox bacterium: A complexome analysis. Biochim Biophys Acta, 1857(10):1694-704(2016) [pubmed]

curator keyword: Biological

submitter keyword: Anammox, electron transport system, complexome profiling, membrane protein complexes

Prof. Dr. Alain J van Gool
contact affiliation	Radboud Proteomics Center, Translational Metabolic Laboratory, Department of Laboratory Medicine, Radboudumc
contact email	Alain.vanGool@radboudumc.nl
lab head
Hans Wessels
contact affiliation	Radboudumc
contact email	hans.wessels@radboudumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD004566

PRIDE project URI

• PRIDE
  1. PXD004566
     1. Label: PRIDE project
     2. Name: Membrane-bound electron transport systems of an anammox bacterium: a complexome analysis