PXD004548

PXD004548 is an original dataset announced via ProteomeXchange.

Title	Transcriptional control of lysosomal homeostasis by the disease-related CLN3 membrane protein
Description	CLN3 is a type II transmembrane protein localized in the late endosomal/lysosomal compartment. A deficiency of CLN3 leads to the development of a certain type of Neuronal Ceroid Lipofuscinosis, a neurodegenerative disorder of childhood caused by aggregation of undegraded material in the lysosomal compartment. Cultured, immortalized wild type and Cln3(Δex7/8) cerebellar granule cells were used in this project to determine the influence of Cln3 deficiency on the proteome of the lysosomal compartment. For this purpose, cells were labelled by stable amino acid labelling in cell culture and lysosomes were isolated by magnetic beads.
HostingRepository	PRIDE
AnnounceDate	2019-05-13
AnnouncementXML	Submission_2019-05-13_00:15:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marc Sylvester
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	6x(13)C labeled residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-11 04:16:16	ID requested
⏵ 1	2019-05-13 00:15:32	announced

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: Mouse, Cerbellar Cells, Lysosomal storage disease

Thomas Braulke
contact affiliation	Section Biochemistry, Children’s Hospital, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
contact email	braulke@uke.de
lab head
Marc Sylvester
contact affiliation	University of Bonn, Institute of Biochemistry and Molecular Biology
contact email	msylvest@uni-bonn.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD004548

PRIDE project URI

• PRIDE
  1. PXD004548
     1. Label: PRIDE project
     2. Name: Transcriptional control of lysosomal homeostasis by the disease-related CLN3 membrane protein