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PXD004546

PXD004546 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuman Rheumatoid Arthritis LC-MSMS
DescriptionMalondialdehyde-modified epitopes (MDA-epitopes) can elicit specific autoantibody that expressed oxidative stress arising in rheumatoid arthritis (RA). The purpose of this study was to discover and validate MDA-peptide adducts as novel biomarkers using concanavalin A affinity chromatography, 1D SDS-PAGE, in-gel digestion, and label-free nano-LC-MS, and evaluate levels of serum MDA, MDA-protein adducts, proteins and autoantibody isotypes against an unmodified and MDA-peptide from Taiwanese female patients with RA and healthy controls (HCs). Levels of serum MDA and MDA-protein adducts were significantly higher in RA patients versus HCs. Four differentially expressed novel MDA-peptides were selected that relative modification ratio were 2-fold differences to examine protein levels and assess autoantibodies to MDA-peptides in RA patients compared with HCs. Four of peptides are autoantigens. Furthermore, 4 MDA-peptides can induce more autoantibody isotypes that are statistical significance in RA patients compared to HCs. Serum IgG and IgM against MDA-peptides showed excellent diagnostic performance in discriminating among RA patients and HCs: area under the curve (AUC, 0.96 ~ 0.98), sensitivity (88.9% ~ 97.8%) and specificity (88.9% ~ 100%). Autoantibodies to MDA-epitopes indicate oxidative modifications occurring in RA, and might be useful as clinical markers fro RA diagnosis if further investigated.
HostingRepositoryPRIDE
AnnounceDate2021-02-25
AnnouncementXMLSubmission_2021-02-25_02:22:51.961.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD004546
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterChing-Yu Lin
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListOxidation; Pyridylethyl
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-07-11 02:52:39ID requested
12021-02-25 02:22:52announced
Publication List
Tsai KL, Chang CC, Chang YS, Lu YY, Tsai IJ, Chen JH, Lin SH, Tai CC, Lin YF, Chang HW, Lin CY, Su EC, Isotypes of autoantibodies against novel differential 4-hydroxy-2-nonenal-modified peptide adducts in serum is associated with rheumatoid arthritis in Taiwanese women. BMC Med Inform Decis Mak, 21(1):49(2021) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: Rheumatoid arthritis, Malondialdehyde, Autoantibody isotypes, Serum
Contact List
Ching-Yu Lin
contact affiliationSchool of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University
contact emailcylin@tmu.edu.tw
lab head
Ching-Yu Lin
contact affiliationSchool of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, No.250, Wuxing Street, Taipei 11031, Taiwan.
contact emailcylin@tmu.edu.tw
dataset submitter
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Dataset FTP location
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