PXD004546

PXD004546 is an original dataset announced via ProteomeXchange.

Title	Human Rheumatoid Arthritis LC-MSMS
Description	Malondialdehyde-modified epitopes (MDA-epitopes) can elicit specific autoantibody that expressed oxidative stress arising in rheumatoid arthritis (RA). The purpose of this study was to discover and validate MDA-peptide adducts as novel biomarkers using concanavalin A affinity chromatography, 1D SDS-PAGE, in-gel digestion, and label-free nano-LC-MS, and evaluate levels of serum MDA, MDA-protein adducts, proteins and autoantibody isotypes against an unmodified and MDA-peptide from Taiwanese female patients with RA and healthy controls (HCs). Levels of serum MDA and MDA-protein adducts were significantly higher in RA patients versus HCs. Four differentially expressed novel MDA-peptides were selected that relative modification ratio were 2-fold differences to examine protein levels and assess autoantibodies to MDA-peptides in RA patients compared with HCs. Four of peptides are autoantigens. Furthermore, 4 MDA-peptides can induce more autoantibody isotypes that are statistical significance in RA patients compared to HCs. Serum IgG and IgM against MDA-peptides showed excellent diagnostic performance in discriminating among RA patients and HCs: area under the curve (AUC, 0.96 ~ 0.98), sensitivity (88.9% ~ 97.8%) and specificity (88.9% ~ 100%). Autoantibodies to MDA-epitopes indicate oxidative modifications occurring in RA, and might be useful as clinical markers fro RA diagnosis if further investigated.
HostingRepository	PRIDE
AnnounceDate	2021-02-25
AnnouncementXML	Submission_2021-02-25_02:22:51.961.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD004546
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ching-Yu Lin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Pyridylethyl
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-11 02:52:39	ID requested
⏵ 1	2021-02-25 02:22:52	announced

Tsai KL, Chang CC, Chang YS, Lu YY, Tsai IJ, Chen JH, Lin SH, Tai CC, Lin YF, Chang HW, Lin CY, Su EC, Isotypes of autoantibodies against novel differential 4-hydroxy-2-nonenal-modified peptide adducts in serum is associated with rheumatoid arthritis in Taiwanese women. BMC Med Inform Decis Mak, 21(1):49(2021) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Rheumatoid arthritis, Malondialdehyde, Autoantibody isotypes, Serum

Ching-Yu Lin
contact affiliation	School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University
contact email	cylin@tmu.edu.tw
lab head
Ching-Yu Lin
contact affiliation	School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, No.250, Wuxing Street, Taipei 11031, Taiwan.
contact email	cylin@tmu.edu.tw
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD004546

PRIDE project URI

• PRIDE
  1. PXD004546
     1. Label: PRIDE project
     2. Name: Human Rheumatoid Arthritis LC-MSMS