PXD004546 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Human Rheumatoid Arthritis LC-MSMS |
Description | Malondialdehyde-modified epitopes (MDA-epitopes) can elicit specific autoantibody that expressed oxidative stress arising in rheumatoid arthritis (RA). The purpose of this study was to discover and validate MDA-peptide adducts as novel biomarkers using concanavalin A affinity chromatography, 1D SDS-PAGE, in-gel digestion, and label-free nano-LC-MS, and evaluate levels of serum MDA, MDA-protein adducts, proteins and autoantibody isotypes against an unmodified and MDA-peptide from Taiwanese female patients with RA and healthy controls (HCs). Levels of serum MDA and MDA-protein adducts were significantly higher in RA patients versus HCs. Four differentially expressed novel MDA-peptides were selected that relative modification ratio were 2-fold differences to examine protein levels and assess autoantibodies to MDA-peptides in RA patients compared with HCs. Four of peptides are autoantigens. Furthermore, 4 MDA-peptides can induce more autoantibody isotypes that are statistical significance in RA patients compared to HCs. Serum IgG and IgM against MDA-peptides showed excellent diagnostic performance in discriminating among RA patients and HCs: area under the curve (AUC, 0.96 ~ 0.98), sensitivity (88.9% ~ 97.8%) and specificity (88.9% ~ 100%). Autoantibodies to MDA-epitopes indicate oxidative modifications occurring in RA, and might be useful as clinical markers fro RA diagnosis if further investigated. |
HostingRepository | PRIDE |
AnnounceDate | 2021-02-25 |
AnnouncementXML | Submission_2021-02-25_02:22:51.961.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD004546 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Ching-Yu Lin |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Oxidation; Pyridylethyl |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-07-11 02:52:39 | ID requested | |
⏵ 1 | 2021-02-25 02:22:52 | announced | |
Publication List
Tsai KL, Chang CC, Chang YS, Lu YY, Tsai IJ, Chen JH, Lin SH, Tai CC, Lin YF, Chang HW, Lin CY, Su EC, Isotypes of autoantibodies against novel differential 4-hydroxy-2-nonenal-modified peptide adducts in serum is associated with rheumatoid arthritis in Taiwanese women. BMC Med Inform Decis Mak, 21(1):49(2021) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Rheumatoid arthritis, Malondialdehyde, Autoantibody isotypes, Serum |
Contact List
Ching-Yu Lin |
contact affiliation | School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University |
contact email | cylin@tmu.edu.tw |
lab head | |
Ching-Yu Lin |
contact affiliation | School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, No.250, Wuxing Street, Taipei 11031, Taiwan. |
contact email | cylin@tmu.edu.tw |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004546
- Label: PRIDE project
- Name: Human Rheumatoid Arthritis LC-MSMS