This dataset consists of 196 raw MS files and associated peak lists and result files, all acquired on a Velos Orbitrap mass spectrometer. Three datasets are associated with this manuscript by St-Denis et al. that describes the interactome for human phosphatases and the function of phosphatases in mitosis. Note that the negative controls and the myotubularin data were also submitted separately as part of a publication on the Myotubularin interactome (St-Denis et al., Mol Cell Proteomics, 2015; PMID 25659891) and assigned to MassIVE ID MSV000078915. See the README file within Methods and Protocols and the Supplementary File description and peptide and interaction identification results in Supplementary Files.