This dataset consists of 145 raw MS files and associated peak lists and result files, all acquired on a Thermo Velos Orbitrap mass spectrometer. Three datasets are associated with this manuscript by St-Denis et al. that describes the interactome for human phosphatases and the function of phosphatases in mitosis. See the README file within Methods and Protocols and the Supplementary File description and peptide and interaction identification results in Supplementary Files.