PXD004523

PXD004523 is an original dataset announced via ProteomeXchange.

Title	Metabolic reconstruction of lipid metabolism of Malassezia
Description	Malassezia species are lipophilic and lipid dependent yeasts belonging to the human and animal microbiota. Typically, they are isolated from regions rich in sebaceous glands. They have been associated with dermatological diseases such as seborrheic dermatitis, tinea versicolor, atopic dermatitis, and folliculitis. Genome sequences of Malassezia globosa, Malassezia sympodialis, and Malassezia pachydermatis lack genes related to fatty acid synthesis. Here, lipid synthesis pathways of M. furfur, M. pachydermatis, M. globosa, M. sympodialis and an atypical variant of M. furfur were reconstructed using genome data and Constraints Based Reconstruction and Analysis. The metabolic reconstruction allowed us to predict variation in the fluxes of each reaction over the network to satisfy the biomass objective function. Proteomic profiling improved and validated the models through data integration. Results suggest that several mechanisms including steroid and butanoate metabolism explain the yeast’s growth under different lipid conditions. Flux differences were observed in production of riboflavin in M. furfur and the biosynthesis of glycerolipids in the atypical variant of M. furfur and Malassezia sympodialis.
HostingRepository	PRIDE
AnnounceDate	2017-10-02
AnnouncementXML	Submission_2017-10-02_08:52:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sergio Triana
SpeciesList	scientific name: Malassezia sympodialis; NCBI TaxID: 76777; scientific name: Malassezia furfur; NCBI TaxID: 55194; scientific name: Malassezia globosa; NCBI TaxID: 76773; scientific name: Malassezia pachydermatis; NCBI TaxID: 77020;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-06 07:14:13	ID requested
⏵ 1	2017-10-02 08:52:42	announced

Triana S, de Cock H, Ohm RA, Danies G, W, ö, sten HAB, Restrepo S, Gonz, á, lez Barrios AF, Celis A, spp. Yeasts Studied through Metabolic Modeling. Front Microbiol, 8():1772(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Malassezia globosa,Malassezia Pachydermatis, Malassezia Furfur,Malassezia Sympodialis

Adriana Celis
contact affiliation	LAMFU, Biological Science department,universidad de los Andes,Colombia Molecular microbiology,Utrecht University, The Netherlands
contact email	acelis@uniandes.edu.co
lab head
Sergio Triana
contact affiliation	Universidad de los Andes
contact email	sh.triana1709@uniandes.edu.co
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004523
     1. Label: PRIDE project
     2. Name: Metabolic reconstruction of lipid metabolism of Malassezia