PXD004520

PXD004520 is an original dataset announced via ProteomeXchange.

Title	Phagosome proteome of activated murine macrophage
Description	Proteomics of carboxylated polystyrene bead (1.0 um) phagosomes from murine bone marrow-derived macrophages. cells were either resting or treated with 100 U/ml IFN-γ (PeproTech) and 100 ng/ml LPS (Sigma) for 24 h, 20 ng/ml Interleukin-4 (IL4) (BD Pharmingen) for 48 h, 20 ng/ml Interleukin-13 (IL13), 10 ng/ml Interleukin-10 (IL10)for 48 h or Reprogrammed (IL4 was incubated with BMDMs for 24 h, and the medium was replaced with fresh medium containing IFN-γ/LPS to incubate for another 24 h). Phagosomes were isolated after 30 min bead inoculation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:30:45.401.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthias Trost
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-06 01:36:29	ID requested
1	2019-03-22 04:51:51	announced
⏵ 2	2024-10-22 04:30:45	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological, Biomedical

submitter keyword: LPS, IL13, endosome, IL10, phagosome,mouse, macrophage, cytokine, reprogrammed, lysosome, Interleukin-4, Interleukin-10, Interleukin-13, Interferon-gamma, IL4

Matthias Trost
contact affiliation	MRC Protein Phosphorylation and Ubiquitylation Unit,School of Life Sciences, University of Dundee, Dundee, Scotland, UK
contact email	m.trost@dundee.ac.uk
lab head
Matthias Trost
contact affiliation	Newcastle University
contact email	matthias.trost@ncl.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD004520

PRIDE project URI

• PRIDE
  1. PXD004520
     1. Label: PRIDE project
     2. Name: Phagosome proteome of activated murine macrophage