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PXD004519

PXD004519 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMudPIT analysis of HSV-1 infected and mock-infected microsomal membranes and nuclear envelopes
DescriptionNuclear envelopes (NEs) and microsomal membranes (MMs) were isolated from HeLa cells infected or not with HSV-1 using well-established procedures as described in: Wilkie GS, et al. (2011) Mol Cell Proteomics 10: M110 003129. Korfali N, et al. (2010) Mol Cell Proteomics 9: 2571-2585. Florens L, Korfali N, Schirmer EC (2008) Methods Mol Biol 432: 117-137. Membrane pellets were denatured, reduced, alkylated then digested with endoproteinase LysC followed by trypsin. Peptides were analyzed by Multidimensional Protein Identification Technology (MudPIT) as described in: Florens L, Washburn MP (2006) Methods Mol Biol 328: 159-175. RAW files were extracted into ms2 file format using RawDistiller v. 1.0 as in: Zhang, Y.; Wen, Z.; Washburn, M. P.; Florens, L. (2011) Anal Chem, 83, (24), 9344-51. MS/MS spectra were queried for peptide sequence information using SEQUEST v.27 (rev.9) as in: Eng J, McCormack A, Yates Jr (1994) J Am Soc Mass Spectrom 5: 976-989. Results from different runs were compared using DTASelect and CONTRAST as in: Tabb DL, McDonald WH, Yates JR (2002) J Proteome Res 1: 21-26. To estimate relative protein levels, distributed normalized spectral abundance factors (dNSAFs) were calculated for each non-redundant protein as in: Zhang Y, Wen Z, Washburn MP, Florens L (2010) Anal Chem 82: 2272-2281.
HostingRepositoryMassIVE
AnnounceDate2019-01-31
AnnouncementXMLSubmission_2019-01-31_07:56:38.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLaurence Florens
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Herpes simplex virus (type 1 / strain 17); NCBI TaxID: 10299;
ModificationListS-carboxamidomethyl-L-cysteine
InstrumentLTQ
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-07-05 07:55:43ID requested
12019-01-31 07:56:38announced
Publication List
no publication
Keyword List
submitter keyword: nuclear envelope, herpesvirus, nuclear egress, vesicle fusion protein
Contact List
Laurence Florens
lab head
Laurence Florens
contact affiliationStowers Institute for Medical Research
contact emaillaf@stowers.org
dataset submitter
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