PXD004519

PXD004519 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	MudPIT analysis of HSV-1 infected and mock-infected microsomal membranes and nuclear envelopes
Description	Nuclear envelopes (NEs) and microsomal membranes (MMs) were isolated from HeLa cells infected or not with HSV-1 using well-established procedures as described in: Wilkie GS, et al. (2011) Mol Cell Proteomics 10: M110 003129. Korfali N, et al. (2010) Mol Cell Proteomics 9: 2571-2585. Florens L, Korfali N, Schirmer EC (2008) Methods Mol Biol 432: 117-137. Membrane pellets were denatured, reduced, alkylated then digested with endoproteinase LysC followed by trypsin. Peptides were analyzed by Multidimensional Protein Identification Technology (MudPIT) as described in: Florens L, Washburn MP (2006) Methods Mol Biol 328: 159-175. RAW files were extracted into ms2 file format using RawDistiller v. 1.0 as in: Zhang, Y.; Wen, Z.; Washburn, M. P.; Florens, L. (2011) Anal Chem, 83, (24), 9344-51. MS/MS spectra were queried for peptide sequence information using SEQUEST v.27 (rev.9) as in: Eng J, McCormack A, Yates Jr (1994) J Am Soc Mass Spectrom 5: 976-989. Results from different runs were compared using DTASelect and CONTRAST as in: Tabb DL, McDonald WH, Yates JR (2002) J Proteome Res 1: 21-26. To estimate relative protein levels, distributed normalized spectral abundance factors (dNSAFs) were calculated for each non-redundant protein as in: Zhang Y, Wen Z, Washburn MP, Florens L (2010) Anal Chem 82: 2272-2281.
HostingRepository	MassIVE
AnnounceDate	2019-01-31
AnnouncementXML	Submission_2019-01-31_07:56:38.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laurence Florens
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Herpes simplex virus (type 1 / strain 17); NCBI TaxID: 10299;
ModificationList	S-carboxamidomethyl-L-cysteine
Instrument	LTQ

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-05 07:55:43	ID requested
⏵ 1	2019-01-31 07:56:38	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: nuclear envelope, herpesvirus, nuclear egress, vesicle fusion protein

submitter keyword: nuclear envelope, herpesvirus, nuclear egress, vesicle fusion protein

Contact List

Laurence Florens
lab head
Laurence Florens
contact affiliation	Stowers Institute for Medical Research
contact email	laf@stowers.org
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000079886