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PXD004509

PXD004509 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCharacterization of the pyrenoid proteome in the green microalga Chlamydomonas reinhardtii
DescriptionThe pyrenoid, a single microcompartment of the chloroplasts of most algae and hornworts, is the major site of CO2 fixation. To compensate the limited availability of CO2 in aquatic environments due to slower CO2 diffusion in water than air, the RubisCO-containing pyrenoid is involved in a carbon concentrating mechanism (CCM) and promotes the carboxylase activity rather than the oxygenase activity of RubisCO, the Calvin-Benson cycle enzyme catalyzing the first step of CO2 fixation. Data in the literature suggest that pyrenoid can have other functions in addition to CO2 fixation. To decipher its functions, the characterization of the pyrenoid proteome in the microalga Chlamydomonas reinhardtii was undertaken. Pyrenoid-enriched fractions from two independent biological cultures (sample1 and sample2) were obtained from cells (WT-cell) or isolated chloroplasts (WT-cp) either from the wild-type (WT) strain or from pyrenoid-deficient Chlamydomonas strains (MX-CW15 and SS-AT) and analyzed by nLC-MS/MS. Substractive proteomic analysis allowed the identification of contaminant proteins and the characterization of the pyrenoid proteome.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:39:27.066.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristophe H. MARCHAND
SpeciesList scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-07-04 06:04:05ID requested
12018-02-28 05:52:55announced
22024-10-22 04:39:33announced2024-10-22: Updated project metadata.
Publication List
10.1371/journal.pone.0185039;
Zhan Y, Marchand CH, Maes A, Mauries A, Sun Y, Dhaliwal JS, Uniacke J, Arragain S, Jiang H, Gold ND, Martin VJJ, Lemaire SD, Zerges W, Pyrenoid functions revealed by proteomics in Chlamydomonas reinhardtii. PLoS One, 13(2):e0185039(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: proteomics,Pyrenoid, carbon fixation, Chlamydomonas reinhardtii
Contact List
Stéphane D. Lemaire
contact affiliationInstitut de Biologie Physico-Chimique, UMR8226, CNRS, Sorbonne Universités, 13 rue Pierre et Marie Curie, 75005 Paris
contact emailstephane.lemaire@ibpc.fr
lab head
Christophe H. MARCHAND
contact affiliationInstitut de Biologie Physico-Chimique, UMR8226, CNRS, Sorbonne Universités, UPMC Univ Paris 06, 13 rue Pierre et Marie Curie, 75005 Paris
contact emailchristophe.marchand@ibpc.fr
dataset submitter
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Dataset FTP location
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PRIDE project URI
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