PXD004509

PXD004509 is an original dataset announced via ProteomeXchange.

Title	Characterization of the pyrenoid proteome in the green microalga Chlamydomonas reinhardtii
Description	The pyrenoid, a single microcompartment of the chloroplasts of most algae and hornworts, is the major site of CO2 fixation. To compensate the limited availability of CO2 in aquatic environments due to slower CO2 diffusion in water than air, the RubisCO-containing pyrenoid is involved in a carbon concentrating mechanism (CCM) and promotes the carboxylase activity rather than the oxygenase activity of RubisCO, the Calvin-Benson cycle enzyme catalyzing the first step of CO2 fixation. Data in the literature suggest that pyrenoid can have other functions in addition to CO2 fixation. To decipher its functions, the characterization of the pyrenoid proteome in the microalga Chlamydomonas reinhardtii was undertaken. Pyrenoid-enriched fractions from two independent biological cultures (sample1 and sample2) were obtained from cells (WT-cell) or isolated chloroplasts (WT-cp) either from the wild-type (WT) strain or from pyrenoid-deficient Chlamydomonas strains (MX-CW15 and SS-AT) and analyzed by nLC-MS/MS. Substractive proteomic analysis allowed the identification of contaminant proteins and the characterization of the pyrenoid proteome.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:39:27.066.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christophe H. MARCHAND
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-04 06:04:05	ID requested
1	2018-02-28 05:52:55	announced
⏵ 2	2024-10-22 04:39:33	announced	2024-10-22: Updated project metadata.

10.1371/journal.pone.0185039;

Zhan Y, Marchand CH, Maes A, Mauries A, Sun Y, Dhaliwal JS, Uniacke J, Arragain S, Jiang H, Gold ND, Martin VJJ, Lemaire SD, Zerges W, Pyrenoid functions revealed by proteomics in Chlamydomonas reinhardtii. PLoS One, 13(2):e0185039(2018) [pubmed]

curator keyword: Biological

submitter keyword: proteomics,Pyrenoid, carbon fixation, Chlamydomonas reinhardtii

Stéphane D. Lemaire
contact affiliation	Institut de Biologie Physico-Chimique, UMR8226, CNRS, Sorbonne Universités, 13 rue Pierre et Marie Curie, 75005 Paris
contact email	stephane.lemaire@ibpc.fr
lab head
Christophe H. MARCHAND
contact affiliation	Institut de Biologie Physico-Chimique, UMR8226, CNRS, Sorbonne Universités, UPMC Univ Paris 06, 13 rue Pierre et Marie Curie, 75005 Paris
contact email	christophe.marchand@ibpc.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/02/PXD004509

PRIDE project URI

• PRIDE
  1. PXD004509
     1. Label: PRIDE project
     2. Name: Characterization of the pyrenoid proteome in the green microalga Chlamydomonas reinhardtii