PXD004509 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Characterization of the pyrenoid proteome in the green microalga Chlamydomonas reinhardtii |
Description | The pyrenoid, a single microcompartment of the chloroplasts of most algae and hornworts, is the major site of CO2 fixation. To compensate the limited availability of CO2 in aquatic environments due to slower CO2 diffusion in water than air, the RubisCO-containing pyrenoid is involved in a carbon concentrating mechanism (CCM) and promotes the carboxylase activity rather than the oxygenase activity of RubisCO, the Calvin-Benson cycle enzyme catalyzing the first step of CO2 fixation. Data in the literature suggest that pyrenoid can have other functions in addition to CO2 fixation. To decipher its functions, the characterization of the pyrenoid proteome in the microalga Chlamydomonas reinhardtii was undertaken. Pyrenoid-enriched fractions from two independent biological cultures (sample1 and sample2) were obtained from cells (WT-cell) or isolated chloroplasts (WT-cp) either from the wild-type (WT) strain or from pyrenoid-deficient Chlamydomonas strains (MX-CW15 and SS-AT) and analyzed by nLC-MS/MS. Substractive proteomic analysis allowed the identification of contaminant proteins and the characterization of the pyrenoid proteome. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:39:27.066.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christophe H. MARCHAND |
SpeciesList | scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-07-04 06:04:05 | ID requested | |
1 | 2018-02-28 05:52:55 | announced | |
⏵ 2 | 2024-10-22 04:39:33 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1371/journal.pone.0185039; |
Zhan Y, Marchand CH, Maes A, Mauries A, Sun Y, Dhaliwal JS, Uniacke J, Arragain S, Jiang H, Gold ND, Martin VJJ, Lemaire SD, Zerges W, Pyrenoid functions revealed by proteomics in Chlamydomonas reinhardtii. PLoS One, 13(2):e0185039(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: proteomics,Pyrenoid, carbon fixation, Chlamydomonas reinhardtii |
Contact List
Stéphane D. Lemaire |
contact affiliation | Institut de Biologie Physico-Chimique, UMR8226, CNRS, Sorbonne Universités, 13 rue Pierre et Marie Curie, 75005 Paris |
contact email | stephane.lemaire@ibpc.fr |
lab head | |
Christophe H. MARCHAND |
contact affiliation | Institut de Biologie Physico-Chimique, UMR8226, CNRS, Sorbonne Universités, UPMC Univ Paris 06, 13 rue Pierre et Marie Curie, 75005 Paris |
contact email | christophe.marchand@ibpc.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004509
- Label: PRIDE project
- Name: Characterization of the pyrenoid proteome in the green microalga Chlamydomonas reinhardtii