PXD004466

PXD004466 is an original dataset announced via ProteomeXchange.

Title	Cellular Ig sequencing
Description	The immune system produces a vast repertoire of immunoglobulins (Ig) in response to the wide number of different antigens to which we are exposed. Thanks to processes such as V(D)J recombination, somatic hypermutation, and antigen selection, the immune system is able of generating the high diversity of Ig sequences. Igs are present in the body as soluble or membrane-bound proteins attached to the B-cells (similar structure to IgM) for regulating the immune system and performing the function of B-cell receptors (BCR), respectively. Specifically, the disrupted activation of the BCR appears as the responsible of the chronic lymphocytic leukemia (CLL). We have focused this study in the sequencing of cellular Ig from B cells purified from the peripheral blood of CLL patients in order to characterize their peptide profiles.
HostingRepository	PRIDE
AnnounceDate	2017-05-08
AnnouncementXML	Submission_2017-05-08_02:26:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paula Díez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-29 03:50:21	ID requested
⏵ 1	2017-05-08 02:26:14	announced

D, í, ez P, Ibarrola N, D, é, gano RM, L, é, crevisse Q, Rodriguez-Caballero A, Criado I, Nieto WG, G, ó, ngora R, Gonz, á, lez M, Almeida J, Orfao A, Fuentes M, A systematic approach for peptide characterization of B-cell receptor in chronic lymphocytic leukemia cells. Oncotarget, 8(26):42836-42846(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: immunoglobulin, BCR, nUPLC-MS/MS

Manuel Fuentes
contact affiliation	Lab 11. Cancer Research Center (CIC). Department of Medicine. USAL. Salamanca. Spain.
contact email	mfuentes@usal.es
lab head
Paula Díez
contact affiliation	Cancer Research Institute-USAL
contact email	pauladg@usal.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD004466

PRIDE project URI

• PRIDE
  1. PXD004466
     1. Label: PRIDE project
     2. Name: Cellular Ig sequencing