PXD004464

PXD004464 is an original dataset announced via ProteomeXchange.

Title	Alterations in kernel proteome after infection with Fusarium culmorum in two triticale cultivars with contrasting resistance to Fusarium head blight
Description	Triticale was used as a model to recognize new components of molecular mechanism of resistance to Fusarium head blight (FHB) in cereals. Fusarium-damaged kernels of two lines distinct in levels of resistance to FHB were applied into a proteome profiling using two-dimensional gel electrophoresis (2-DE) to create protein maps and mass spectrometry to identify the proteins differentially accumulated between the analyzed lines. The 2-DE analysis indicated a total of 23 spots with clear differences in a protein content between the more resistant and more susceptible triticale lines after infection with F. culmorum. A majority of the proteins were involved in a cell carbohydrate metabolism, stressing the importance of this protein group in a plant response to Fusarium infection. The increased accumulation levels of different isoforms of plant beta-amylase were observed for a more susceptible triticale line after inoculation. The more resistant line was characterized by a higher abundance of alpha-amylase inhibitor CM2 subunit.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:31:07.968.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Agata Malinowska
SpeciesList	scientific name: Secale cereale x Triticum turgidum subsp. durum; NCBI TaxID: 142809;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-29 03:25:41	ID requested
1	2018-10-26 15:31:33	announced
⏵ 2	2024-10-22 04:31:12	announced	2024-10-22: Updated project metadata.

Perlikowski D, Wi, ś, niewska H, Kaczmarek J, G, ó, ral T, Ochodzki P, Kwiatek M, Majka M, Augustyniak A, Kosmala A, Alterations in Kernel Proteome after Infection with Fusarium culmorum in Two Triticale Cultivars with Contrasting Resistance to Fusarium Head Blight. Front Plant Sci, 7():1217(2016) [pubmed]

10.3389/fpls.2016.01217;

curator keyword: Biological

submitter keyword: leaf, triticale,LC-MSMS

Michal Dadlez
contact affiliation	Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
contact email	michald@ibb.waw.pl
lab head
Agata Malinowska
contact affiliation	IBB PAS
contact email	esme@ibb.waw.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD004464

PRIDE project URI

• PRIDE
  1. PXD004464
     1. Label: PRIDE project
     2. Name: Alterations in kernel proteome after infection with Fusarium culmorum in two triticale cultivars with contrasting resistance to Fusarium head blight