PXD004457

PXD004457 is an original dataset announced via ProteomeXchange.

Title	Shotgun proteomics analysis of Desulfovibrio alaskensis in response to Platinum and Palladium.
Description	Platinum and palladium are highly sought-after noble metals that due to their low abundance have high value and because of their stability and their roles in catalytic processes are very desirable for industrial purposes. Bacteria are able to produce nanoparticles of platinum and palladium at low temperatures and from low concentration feedstocks contrary to chemical methods and so pose a potentially untapped ‘green’ resource for nanoparticle synthesis. We have used the bacterium Desulfovibrio alaskensis G20 to reduce Pt and Pd ions to zero-valent nanoparticle forms and used differential shotgun proteomics to identify proteins responsible for this reduction . There was found to be a core set of 13 proteins common to both datasets as well as 7 proteins specific to Pt and Pd individually. Over expression of one of Pt-specific genes; the NiFe hydrogenase small subunit, resulted in the formation of larger nanoparticles. For the first time the proteins involved in the metal reduction pathway have been pinpointed and it is our hope that these target genes can then be used for nanoparticle production to tailor specific properties for industrial purposes at the genetic level rather than post-production.
HostingRepository	PRIDE
AnnounceDate	2022-02-22
AnnouncementXML	Submission_2022-02-22_03:49:43.404.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD004457
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Thierry Le Bihan
SpeciesList	scientific name: Desulfovibrio alaskensis G20; NCBI TaxID: 207559;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-28 06:41:21	ID requested
⏵ 1	2022-02-22 03:49:44	announced

Capeness MJ, Imrie L, M, ü, hlbauer LF, Le Bihan T, Horsfall LE, in response to platinum and palladium. Microbiology (Reading), 165(12):1282-1294(2019) [pubmed]

curator keyword: Biological

submitter keyword: Desulfovibrio alaskensis, Proteomics, label-free, nanoparticles, Platinum, Palladium

Thierry Le Bihan
contact affiliation	Dr Thierry Le Bihan Quantitative Proteomics lecturer and technology lead Institute of Structural and Molecular Biology SynthSys – Synthetic and Systems Biology School of Biological Sciences, University of Edinburgh Room 3.06, C.H Waddington Building, Max Born Crescent Edinburgh, EH9 3BF Phone +44 (0)131 651 9073 thierry.lebihan@ed.ac.uk
contact email	thierry.lebihan@ed.ac.uk
lab head
Thierry Le Bihan
contact affiliation	SynthSys – Synthetic and Systems Biology
contact email	thierry.lebihan@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD004457

PRIDE project URI

• PRIDE
  1. PXD004457
     1. Label: PRIDE project
     2. Name: Shotgun proteomics analysis of Desulfovibrio alaskensis in response to Platinum and Palladium.