PXD004455

PXD004455 is an original dataset announced via ProteomeXchange.

Title	Silkworm spinneret LC-MSMS
Description	Spinneret, which locates at the end of the silk gland, is an important tissue for silk spinning. Although it has been discovered for hundreds of years, its particular roles in silk spinning and fiber formation are still unclear. Here we report the first proteome profile of silkworm spinnerets by LC-MS/MS. Totally, 1572 non-redundant proteins and 232 differential expressed proteins were identified in the spinneret from the spinning larvae and the third day of fifth instar larvae. Silk fiber formation related proteins, such as cuticle proteins, ion-transporting proteins, muscular proteins, and energy metabolic proteins, were abundant in the spinneret. By analyzing the signal pathways, we discovered that the processes associated with energy metabolism were active in spinneret. GO enrichment analysis revealed that the differential expressed proteins were involved in energy metabolism, chitin binding, and cuticle construction. The active energy metabolism might provide abundant energy for the muscle contraction, ion and water transporting. The chitin binding and cuticle construction process might provide sufficient shear forces for silk formation. This dataset suggests that the spinneret provided a suitable physiological and biochemical environment for silk formation, and will be helpful for elucidating the functions of spinneret.
HostingRepository	PRIDE
AnnounceDate	2017-05-08
AnnouncementXML	Submission_2017-05-08_02:28:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xin Wang
SpeciesList	scientific name: Bombyx mori (Silk moth); NCBI TaxID: 7091;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-28 06:15:19	ID requested
⏵ 1	2017-05-08 02:28:41	announced

Wang X, Li Y, Liu Q, Xia Q, Zhao P, Proteome profile of spinneret from the silkworm, Bombyx mori. Proteomics, 17(12):(2017) [pubmed]

curator keyword: Biological

submitter keyword: Silkworm, spinneret, insect tissue, LC-MSMS

Ping Zhao
contact affiliation	State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, PRC
contact email	zhaop@swu.edu.cn
lab head
Xin Wang
contact affiliation	State Key Laboratory of Silkworm Genome Biology, Southwest Univ., China
contact email	wangxin1-4@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD004455

PRIDE project URI

• PRIDE
  1. PXD004455
     1. Label: PRIDE project
     2. Name: Silkworm spinneret LC-MSMS