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PXD004452

PXD004452 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHeLa proteome of 12,250 protein-coding genes
DescriptionWe developed an optimized multi-shot proteomics workflow based on high-resolution offline high pH reversed-phase peptide separation of high peptide loads collecting many fractions that were in turn analyzed by short online chromatographic separations and fast peptide sequencing using orbitrap tandem mass spectrometry.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:30:37.606.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristian Kelstrup
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonomethylated residue; phosphorylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-06-28 01:56:01ID requested
12017-06-12 07:29:13announced
22024-10-22 04:30:42announced2024-10-22: Updated project metadata.
Publication List
Bekker-Jensen DB, Kelstrup CD, Batth TS, Larsen SC, Haldrup C, Bramsen JB, S, ø, rensen KD, H, ø, yer S, Ø, rntoft TF, Andersen CL, Nielsen ML, Olsen JV, An Optimized Shotgun Strategy for the Rapid Generation of Comprehensive Human Proteomes. Cell Syst, 4(6):587-599.e4(2017) [pubmed]
10.1016/j.cels.2017.05.009;
Keyword List
ProteomeXchange project tag: deep learning, benchmarking, machine learning, immunopeptidomics
curator keyword: Technical, Biological
submitter keyword: hela, tissue, deep proteomes, cell lines,shotgun proteomics
Contact List
Jesper V. Olsen
contact affiliationNovo Nordisk Foundation Center for Protein Research, University of Copenhagen, Denmark
contact emailjesper.olsen@cpr.ku.dk
lab head
Christian Kelstrup
contact affiliationUniversity of Copenhagen
contact emailchdake@cpr.ku.dk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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