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PXD004430

PXD004430 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSecretome profiling of human airway epithelial cultures infected with respiratory syncytial virus
DescriptionThe respiratory epithelium is a polarised layer at the interface between the outside environment and deeper lung structures, overlaid by the epithelial lining fluid (ELF). This provides a mechanical and immunological barrier to inhaled particulates, such as viruses. Human respiratory syncytial virus (hRSV) is a major cause of disease in humans, and targets the respiratory epithelium. However, little is known of the disruption of the ELF proteome in the context of virus-driven respiratory illnesses. To address this, a proteomics approach was combined with an ex-vivo human airway epithelial model (HAE) to investigate the apical and basolateral secretome in hRSV-infected cultures. This demonstrated that several apically- and basolaterally-restricted proteins were subsequently secreted in both directions upon infection, while a number of proteins saw their apical/basolateral abundance ratios significantly altered. Furthermore, another 35 proteins were uniquely identified after hRSV treatment. Importantly, some of these changes were correlated in nasal aspirates (NA) from children with and without hRSV. This study showed that hRSV could affect airway secretions, and disrupted the directionality of the respiratory epithelium.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:34:34.368.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterStuart Armstrong
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-06-23 08:31:56ID requested
12022-03-01 08:33:49announced
22024-10-22 05:34:40announced2024-10-22: Updated project metadata.
Publication List
10.1074/mcp.ra119.001546;
Touzelet O, Broadbent L, Armstrong SD, Aljabr W, Cloutman-Green E, Power UF, Hiscox JA, The Secretome Profiling of a Pediatric Airway Epithelium Infected with hRSV Identified Aberrant Apical/Basolateral Trafficking and Novel Immune Modulating (CXCL6, CXCL16, CSF3) and Antiviral (CEACAM1) Proteins. Mol Cell Proteomics, 19(5):793-807(2020) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: Human respiratory syncytial virus, human airway epithelial model, secretome, LC-MS/MS
Contact List
Julian Hiscox
contact affiliationChair in Infection and Global Health, Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool,
contact emailJulian.Hiscox@liverpool.ac.uk
lab head
Stuart Armstrong
contact affiliationInfection Biology
contact emailsarmstro@liv.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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