PXD004428

PXD004428 is an original dataset announced via ProteomeXchange.

Title	Microbial functionality as affected by experimental warming of a temperate mountain forest soil - a metaproteomics survey
Description	Soils are a huge reservoir of organic C, and the efflux of CO2 from soils is one of the largest fluxes in the global C cycle. Out of all natural environments, soils probably contain the greatest microbial biomass and diversity, which classifies them as one of the most challenging habitats for microbiologists (Mocali and Benedetti, 2010). Until today, it is not well understood how soil microorganisms will respond to a warmer climate. Warming may give competitive advantage to species adapted to higher temperatures (Rinnan et al., 2009). The mechanisms behind temperature adaptations of soil microbes could be shifts within the microbial community. How microbial communities will ultimately respond to climate change, however, is still a matter of speculation. As a post-genomic approach in nature, metaproteomics allows the simultaneous examination of various protein functions and responses, and therefore is perfectly suited to investigate the complex interplay between respiration dynamics, microbial community architecture, and ecosystem functioning in a changing environment (Bastida et al., 2012). Thereby we will gain new insights into responses to climate change from a microbial perspective. Our study site was located at 910 m a.s.l. in the North Tyrolean Limestone Alps, near Achenkirch, Austria The 130 year-old mountain forests consist of Norway spruce (Picea abies) with inter-spread of European beech (Fagus sylvatica) and silver fir (Abies alba). Three experimental plots with 2 × 2 m warmed- and control- subplots were installed in 2004. The temperature difference between control and warmed plots was set to 4 °C at 5 cm soil depth. Soil was warmed during snow-free seasons. In order to extract proteins from forest soil samples, the SDS–phenol method was adopted as previously described by Keiblinger et al. (2012). Protein extractions were performed from each subplot soil samples. The abundance of protein-assigned microbial phylogenetic and functional groups, were calculated based on the normalized spectral abundance factor (NSAF, Zybailov et al., 2006).
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:49:09.796.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD004428
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dong Liu
SpeciesList	scientific name: uncultured soil microorganism; NCBI TaxID: 1457551;
ModificationList	Oxidation
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-23 03:45:11	ID requested
1	2022-03-14 02:38:28	announced
2	2022-03-14 02:40:01	announced	2022-03-14: Updated publication reference for DOI(s): doi.org/10.1016/j.apsoil.2017.04.021.
⏵ 3	2023-11-14 08:49:10	announced	2023-11-14: Updated project metadata.

10.6019/PXD004428;

curator keyword: Metaproteomics, Biological

submitter keyword: soil microbial diversity, microbial community functionality,Soil warming

Katharina M. Keiblinger
contact affiliation	Lab head at the Institute for Soil Research, University of Natural Resources and Applied Life Sciences (BOKU)
contact email	katharina.keiblinger@boku.ac.at
lab head
Dong Liu
contact affiliation	Ph.D
contact email	ld19871015@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD004428

PRIDE project URI

• PRIDE
  1. PXD004428
     1. Label: PRIDE project
     2. Name: Microbial functionality as affected by experimental warming of a temperate mountain forest soil - a metaproteomics survey