PXD004427

PXD004427 is an original dataset announced via ProteomeXchange.

Title	Identification of proteins using iTRAQ and VIGS revealed that three proteins were involved into cold response in bread wheat
Description	iTRAQ-based quantitative proteomic and liquid chromatography tandem mass spectrometry (LC-MS/MS) methods were applied to identify protein spots involved in cold responses in wheat. By comparing the differentially accumulated proteins from the derivatives (UC1110 x PI 610750), as well as the F10 recombinant inbred line (RIL) population differing in cold-tolerance, a total of 223 proteins with significantly altered abundance were identified, respectively. These proteins were classified into several main groups, as follows: protein metabolism, stress/defense, carbohydrate metabolism, lipid metabolism, RNA metabolism, energy production, cell-wall and cytoskeleton metabolism, membrane and transportation, signal transduction, other metabolic processes, and unknown biological processes. The 22 differentially expressed protein spots were chosen for quantitative real-time polymerase chain reaction (qRT-PCR) to investigate expression changes at the RNA level. The results indicated that the transcriptional expression patterns of 10 genes were consistent with their protein expression models. Furthermore, quantitative real-time PCR (qRT-PCR) results implied that abiotic stresses increased the expression of four candidate protein genes Heat shock protein 90 (TaHsp90.2), Bowman-Birk type protease inhibitor (Bowman), REP14, and VER2 in wheat leaves. VIGS (virus-induced gene silencing)-treated plants generated for TaHsp90.2, Bowman, and REP14 were subjected to cold limitation, and had more serious droop and wilt, increased rate of relative electrolyte leakage and reduced relative water content (RWC) when compared to viral control plants. These results indicated that TaHsp90.2, Bowman, and REP14 possibly played the important roles in conferring cold tolerance in wheat. This study could provide useful information for dissection of molecular and genetics basis of cold stress in bread wheat.
HostingRepository	PRIDE
AnnounceDate	2017-08-16
AnnouncementXML	Submission_2017-08-16_00:14:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ning Zhang
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-23 03:27:47	ID requested
⏵ 1	2017-08-16 00:14:45	announced

Zhang N, Zhang L, Zhao L, Ren Y, Cui D, Chen J, Wang Y, Yu P, Chen F, iTRAQ and virus-induced gene silencing revealed three proteins involved in cold response in bread wheat. Sci Rep, 7(1):7524(2017) [pubmed]

curator keyword: Biological

submitter keyword: cold responsive proteins, bread wheat, iTRAQ

Feng Chen
contact affiliation	Agronomy College/National Key Laboratory of Wheat and Corn Crop/Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University ,Zhengzhou China
contact email	chf0088@163.com
lab head
Ning Zhang
contact affiliation	Henan Agricultural University
contact email	zhangning88012@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004427
     1. Label: PRIDE project
     2. Name: Identification of proteins using iTRAQ and VIGS revealed that three proteins were involved into cold response in bread wheat