PXD004423

PXD004423 is an original dataset announced via ProteomeXchange.

Title	milk-derived extracellular vesicles UHPLC-MS
Description	Breast milk contains macromolecular components with distinctive functions, whereby milk fat globules and casein micelles provide nutrition, while whey contains molecules that stimulate the infant’s immunity. Interestingly, the importance and function of extracellular vesicles (EV) in milk has not been addressed in detail. This in part is due to the difficulty to purify EV from other milk components and consequently the molecular composition of human milk-derived EV has not been fully elucidated. Methods: In this study, an extensive LC-MS/MS proteomic analysis was performed on EV isolated via density-based separation from human breast milk of 7 individuals. The proteome of milk-derived EV was analyzed using FunRich and compared to the Vesiclepedia database, as well as to the whole milk proteome, which was constructed from 38 previously published milk proteomics studies.
HostingRepository	PRIDE
AnnounceDate	2016-09-27
AnnouncementXML	Submission_2016-09-27_13:50:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Soenita Goerdayal
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-23 02:18:20	ID requested
⏵ 1	2016-09-27 13:50:16	announced

van Herwijnen MJ, Zonneveld MI, Goerdayal S, Nolte-'t Hoen EN, Garssen J, Stahl B, Maarten Altelaar AF, Redegeld FA, Wauben MH, Comprehensive Proteomic Analysis of Human Milk-derived Extracellular Vesicles Unveils a Novel Functional Proteome Distinct from Other Milk Components. Mol Cell Proteomics, 15(11):3412-3423(2016) [pubmed]

curator keyword: Biological

submitter keyword: Human milk, vesicles, LC-MS, milk proteome

Marca H.M. Wauben
contact affiliation	Department of Biochemistry & Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands
contact email	m.h.m.wauben@uu.nl
lab head
Soenita Goerdayal
contact affiliation	Molecular mass spectrometry and proteomics, Utrecht University, Padualaan 8 3584CH Utrecht, The Netherlands
contact email	s.s.goerdayal@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD004423

PRIDE project URI

• PRIDE
  1. PXD004423
     1. Label: PRIDE project
     2. Name: milk-derived extracellular vesicles UHPLC-MS