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PXD004421

PXD004421 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHypoxia decreases the pathogenicity of Pseudomonas aeruginosa by reducing expression of multiple virulence factors.
DescriptionHypoxia is a feature of the microenvironment during P. aeruginosa infection in several disease states. We confirm that the pathogenicity of P. aeruginosa derived from sites of acute infection is higher than those derived from sites of chronic infection. Hypoxia attenuated the pathogenicity of acute but not chronic strains implicating a role for hypoxia in controlling virulence. Mass spectrometric analysis revealed reduced expression of multiple virulence factors in hypoxia including exotoxin A, alkaline protease and proteins important in the synthesis of pyoverdine. Inhibition of pyoverdine production by iron supplementation mimicked the effects of hypoxia. Finally, strains of P. aeruginosa which lacks a functional pseudomonas prolyl-hydroxylase domain containing protein (PPHD) do not respond to hypoxia implicating a possible role for PPHD as an oxygen-sensing determinant of pathogenicity. Understanding how hypoxia influences bacterial virulence will identify new targets for anti–infective therapy against P. aeruginosa.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:31:00.047.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlex von kriegsheim
SpeciesList scientific name: Pseudomonas aeruginosa str. E2UoS; NCBI TaxID: 1333550;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-06-22 07:46:23ID requested
12017-06-06 07:20:38announced
22024-10-22 04:31:04announced2024-10-22: Updated project metadata.
Publication List
Schaible B, Rodriguez J, Garcia A, von Kriegsheim A, McClean S, Hickey C, Keogh CE, Brown E, Schaffer K, Broquet A, Taylor CT, Hypoxia Reduces the Pathogenicity of Pseudomonas aeruginosa by Decreasing the Expression of Multiple Virulence Factors. J Infect Dis, 215(9):1459-1467(2017) [pubmed]
10.1093/infdis/jix139;
Keyword List
curator keyword: Biomedical
submitter keyword: Hypoxia, Pseudomonas aeruginosa
Contact List
Alex von Kriegsheim
contact affiliationECRC, IGMM, University of Edinburgh
contact emailkriegsheim@gmail.com
lab head
Alex von kriegsheim
contact affiliationUniversity of Edinburgh
contact emailalex.vonkriegsheim@ucd.ie
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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