PXD004421 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Hypoxia decreases the pathogenicity of Pseudomonas aeruginosa by reducing expression of multiple virulence factors. |
Description | Hypoxia is a feature of the microenvironment during P. aeruginosa infection in several disease states. We confirm that the pathogenicity of P. aeruginosa derived from sites of acute infection is higher than those derived from sites of chronic infection. Hypoxia attenuated the pathogenicity of acute but not chronic strains implicating a role for hypoxia in controlling virulence. Mass spectrometric analysis revealed reduced expression of multiple virulence factors in hypoxia including exotoxin A, alkaline protease and proteins important in the synthesis of pyoverdine. Inhibition of pyoverdine production by iron supplementation mimicked the effects of hypoxia. Finally, strains of P. aeruginosa which lacks a functional pseudomonas prolyl-hydroxylase domain containing protein (PPHD) do not respond to hypoxia implicating a possible role for PPHD as an oxygen-sensing determinant of pathogenicity. Understanding how hypoxia influences bacterial virulence will identify new targets for anti–infective therapy against P. aeruginosa. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:31:00.047.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alex von kriegsheim |
SpeciesList | scientific name: Pseudomonas aeruginosa str. E2UoS; NCBI TaxID: 1333550; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-06-22 07:46:23 | ID requested | |
1 | 2017-06-06 07:20:38 | announced | |
⏵ 2 | 2024-10-22 04:31:04 | announced | 2024-10-22: Updated project metadata. |
Publication List
Schaible B, Rodriguez J, Garcia A, von Kriegsheim A, McClean S, Hickey C, Keogh CE, Brown E, Schaffer K, Broquet A, Taylor CT, Hypoxia Reduces the Pathogenicity of Pseudomonas aeruginosa by Decreasing the Expression of Multiple Virulence Factors. J Infect Dis, 215(9):1459-1467(2017) [pubmed] |
10.1093/infdis/jix139; |
Keyword List
curator keyword: Biomedical |
submitter keyword: Hypoxia, Pseudomonas aeruginosa |
Contact List
Alex von Kriegsheim |
contact affiliation | ECRC, IGMM, University of Edinburgh |
contact email | kriegsheim@gmail.com |
lab head | |
Alex von kriegsheim |
contact affiliation | University of Edinburgh |
contact email | alex.vonkriegsheim@ucd.ie |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004421
- Label: PRIDE project
- Name: Hypoxia decreases the pathogenicity of Pseudomonas aeruginosa by reducing expression of multiple virulence factors.