PXD004419

PXD004419 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis uncovers common effects of IFN-γ and IL-27 on antigen presentation machinery components in human cancer cells
Description	IL-27, a member of the IL-12- family of cytokines, has shown anti-tumor activity in several pre-clinical models due to anti-proliferative, anti-angiogenic and immune-enhancing effects. On the other hand, IL-27 demonstrated immune-regulatory activities and inhibition of auto-immunity in mouse models. Also, we reported that IL-27, similar to IFN-, induces the expression of IL-18BP, IDO and PD-L1 immune-regulatory molecules in human cancer cells. Here, a proteomic analysis reveals that IL-27 and IFN- display a broad overlap of functions on human ovarian cancer cells. Indeed, among 990 proteins modulated by either cytokine treatment in SKOV3 cells, 814 showed a concordant modulation by both cytokines, while a smaller number (176) were differentially modulated. The most up-regulated proteins were common to both IFN-γ and IL-27. In addition, functional analysis of IL-27- regulated protein networks highlighted pathways of interferon signaling and regulation, antigen presentation, protection from natural killer cell-mediated cytotoxicity, regulation of protein polyubiquitination and proteasome, aminoacid catabolism and regulation of viral protein levels.
HostingRepository	PRIDE
AnnounceDate	2017-05-19
AnnouncementXML	Submission_2017-05-18_23:50:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrea Petretto
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-22 06:35:04	ID requested
⏵ 1	2017-05-18 23:50:22	announced

Petretto A, Carbotti G, Inglese E, Lavarello C, Pistillo MP, Rigo V, Croce M, Longo L, Martini S, Vacca P, Ferrini S, Fabbi M, and IL-27 on the HLA class I antigen presentation machinery in human cancer cells. Oncotarget, 7(45):72518-72536(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: IL-27, interleukin-27

IFN-γ, interferon gamma

proteomic analysis

HLA class I

APM, antigen presentation machinery.

Andrea Petretto
contact affiliation	Core Facilities, Istituto Giannina Gaslini, Genoa, Italy
contact email	andreapetretto@gaslini.org
lab head
Andrea Petretto
contact affiliation	Gaslini Children's Hospital
contact email	a.petretto@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD004419

PRIDE project URI

• PRIDE
  1. PXD004419
     1. Label: PRIDE project
     2. Name: Proteomic analysis uncovers common effects of IFN-γ and IL-27 on antigen presentation machinery components in human cancer cells