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PXD004413

PXD004413 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProtein turnover measurement using selected reaction monitoring-mass spectrometry (SRM-MS) C4PR_LIV
DescriptionProtein turnover represents an important mechanism in the functioning of cells, with deregulated synthesis and degradation of proteins implicated in many diseased states. Therefore, proteomics strategies to measure, with high confidence, turnover rates are of vital importance to understanding many biological processes. In this study, the more widely used approach of non-targeted precursor ion signal intensity (MS1) quantification is compared to selected reaction monitoring (SRM), a data acquisition strategy that records data for specific peptides, to determine if improved quantitative data would be obtained using a targeted quantification approach. Using mouse liver as a model system, turnover measurement of four tricarboxylic acid cycle proteins was performed using both MS1 and SRM quantification strategies. SRM outperformed MS1 in terms of sensitivity and selectivity of measurement, allowing more confident determination of protein turnover rates. SRM data is acquired using cheaper and more widely available tandem quadrupole mass spectrometers, making the approach accessible to a larger number of researchers than MS1 quantification, which is best performed on high mass resolution instruments. SRM acquisition is ideally suited to focussed studies where the turnover of tens of proteins is measured, making it applicable in determining the dynamics of proteins complexes and complete metabolic pathways.
HostingRepositoryPRIDE
AnnounceDate2017-02-16
AnnouncementXMLSubmission_2017-02-16_00:30:49.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD004413
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterStephen Holman
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListOxidation; Label:13C(6); Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-06-22 01:31:29ID requested
12016-08-10 06:56:46announced
22017-02-16 00:30:49announcedUpdated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: Protein turnover, SRM, MS1, proteomics, quantification, mass spectrometry
Contact List
Robert J. Beynon
contact affiliationCentre for Proteome Research, Department of Biochemistry, University of Liverpool, UK
contact emailr.beynon@liv.ac.uk
lab head
Stephen Holman
contact affiliationUniversity of Liverpool
contact emailstephen.holman@liverpool.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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