PXD004397

PXD004397 is an original dataset announced via ProteomeXchange.

Title	Normal human mitral valve proteome: a preliminary investigation by gel-based and gel-free proteomic approaches
Description	The mitral valve is a highly complex structure which regulates blood flow from the left atrium to the left ventricle (LV) avoiding a significant forward gradient during diastole or regurgitation during systole. The integrity of the mitral valve is also essential for the maintenance of normal LV size, geometry, and function. Significant advances in the comprehension of the biological, functional, and mechanical behavior of the mitral valve have recently been made. However, current knowledge of protein components in the normal human mitral valve is still limited and complicated by the low cellularity of this tissue and the presence of high abundant proteins from the extracellular matrix. We employed here an integrated proteomic approach to analyse the protein composition of the normal human mitral valve and reported confident identification of 422 proteins, some of which have not been previously described in this tissue. In particular, we described the ability of pre-MS separation technique based on liquid-phase IEF and SDS-PAGE to identify the largest number of proteins. These initial results provide a valuable basis for future studies aimed at analysing in depth the mitral valve protein composition and at investigating potential pathogenetic molecular mechanisms.
HostingRepository	PRIDE
AnnounceDate	2016-08-18
AnnouncementXML	Submission_2017-02-13_03:41:27.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD004397
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Maura Brioschi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue: 79.96633; N-acetylhexosaminylated: 203.0794; iodoacetamide derivatized residue: 57.02146; acetylated residue: 42.01056; monohydroxylated residue: 15.99491; deamidated residue: 0.984
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-21 05:40:56	ID requested
⏵ 1	2017-02-13 03:41:28	announced

Brioschi M, Baetta R, Ghilardi S, Gianazza E, Guarino A, Parolari A, Polvani G, Tremoli E, Banfi C, Normal human mitral valve proteome: A preliminary investigation by gel-based and gel-free proteomic approaches. Electrophoresis, 37(20):2633-2643(2016) [pubmed]

curator keyword: Biological

submitter keyword: human normal mitral valve, LC/MSE, valvular interstitial cells

Cristina Banfi
contact affiliation	Centro Cardiologico Monzino, via Parea 4, 20138 Milan, Italy
contact email	cristina.banfi@ccfm.it
lab head
Maura Brioschi
contact affiliation	Laboratory of cellular biology and biochemistry of atherothrombosis
contact email	maura.brioschi@ccfm.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004397
     1. Label: PRIDE project
     2. Name: Normal human mitral valve proteome: a preliminary investigation by gel-based and gel-free proteomic approaches