PXD004395

PXD004395 is an original dataset announced via ProteomeXchange.

Title	Global mapping of rat plasma proteins with a native proteomic approach using nondenaturing micro 2DE and label-free quantitative LC-MS/MS
Description	Plasma samples from adult male rats were separated by nondenaturing micro 2DE and a representative gel was selected, on which a total of 136 CBB-stained spots were numbered and subjected to in-gel digestion and label-free quantitative LC-MSE (MS/MS in data-independent acquisition mode). The analysis provided the assignment of 1-25 (average eight) nonredundant proteins in each spot and totally 199 proteins were assigned in the 136 spots. The quantity data from the LC-MSE analysis were further analyzed to reconstruct the quantity distribution on the 2D gel for each of the assigned proteins (a native protein map). This work provides not only a reference library containing 199 native protein maps for rat plasma proteome, but also useful information for subsequent analysis for protein-protein interactions.
HostingRepository	PRIDE
AnnounceDate	2016-10-14
AnnouncementXML	Submission_2016-10-14_03:03:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ya Jin
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-21 03:53:07	ID requested
⏵ 1	2016-10-14 03:03:42	announced

Chen S, Wen M, Bu S, Wang A, Jin Y, Tan W, Global mapping of rat plasma proteins with a native proteomic approach using nondenaturing micro 2DE and quantitative LC-MS/MS. Electrophoresis, 37(23-24):3126-3136(2016) [pubmed]

curator keyword: Biological

submitter keyword: Native proteomics, Global mapping, Adult male rat plasma proteome, Nondenaturing micro 2DE, Label-free quantitative LC-MSE

Ya Jin
contact affiliation	School of Bioscience & Bioengineering, South China University of Technology
contact email	ya.jin@ymail.com
lab head
Ya Jin
contact affiliation	School of Bioscience and Biotechnology, South China University of Technology
contact email	ya.jin@ymail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004395
     1. Label: PRIDE project
     2. Name: Global mapping of rat plasma proteins with a native proteomic approach using nondenaturing micro 2DE and label-free quantitative LC-MS/MS