PXD004370

PXD004370 is an original dataset announced via ProteomeXchange.

Title	To compare the proteome of Stenotrophomonas maltophilia grown in normal and iron depleted condition
Description	As an approach to identify the iron depleted responsive protein, ITRAQ approach was used to analyze the supernatant of S. maltophilia strains [clinical (CS17) and environmental (LMG959)] grown in iron depleted condition. Appendix A and B lists the up-regulated proteins identified with ≥95% confidence including their respective ratio and peptides. A total of 480 and 358 proteins were identified in replicate 1 and replicate 2 which represent two independent analysis of the experiments for both strains that met the criteria of a minimum unused score cut of >1.3 which indicates >95% confidence in correct sequence identification. Figure 4 showed that 687 proteins were detected in which 151 proteins were commonly present in replicates, 329 and 207 proteins being unique to replicate 1 and replicate 2 respectively. From 687 proteins detected, a total of 122 proteins were seen with altered expression in response to iron depleted condition among both strains. Of these, 36 proteins upregulated and 7 down-regulated in LMG959 while for CS17, 60 proteins upregulated and 19 down-regulated (Appendix A).In another comparison, CS17 and LMG959 grown in normal condition were compared. ITRAQ identified 81 differently regulated proteins with 40 proteins up-regulated and 41 proteins down-regulated in CS17 when compared to LMG959 grown in normal condition.
HostingRepository	PRIDE
AnnounceDate	2020-05-26
AnnouncementXML	Submission_2020-05-26_11:05:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Narcisse Kharthic
SpeciesList	scientific name: Stenotrophomonas maltophilia; NCBI TaxID: 40324;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-20 06:14:35	ID requested
⏵ 1	2020-05-26 11:05:43	announced

V K, Neela VK, , phenotypic and proteomic approaches. Virulence, 11(1):104-112(2020) [pubmed]

curator keyword: Biological

submitter keyword: Stenotrophomonas maltophilia, iron depleted, proteome

Associate Professor Dr. Vasantha Kumari Neela
contact affiliation	Department of Medical Microbiology and Parasitology, Universiti Putra Malaysia, Serdang 43400, Malaysia.
contact email	neela2000@hotmail.com
lab head
Narcisse Kharthic
contact affiliation	Universiti Putra Malaysia
contact email	narcissemsjoseph@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD004370

PRIDE project URI

• PRIDE
  1. PXD004370
     1. Label: PRIDE project
     2. Name: To compare the proteome of Stenotrophomonas maltophilia grown in normal and iron depleted condition