PXD004365

PXD004365 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis of exoproteome and membrane enriched fractions of PAK and PAK pJN2133 strains
Description	This work characterizes a P.aeruginosa strain with low levels of c-di-GMP resultant from the overexpression of the phosphodiesterase PA2133. Phenotypic characterization showed that biofilm formation, adhesion to epitheliel cells, and motility are severely impaired in this strain. We applied both, DIGE / MALDI-TOF/TOF and shotgun LC/MS/MS quantitative proteomic analysis to exoproteome and enriched membrane protein fractions. Results from both complementary strategies were in agreement and revealed motility, type III secretion, and chemotaxis to be the main affected processes by PA2133 overexpression. These results are the first to show that flagella synthesis, twitching motility, and chemotaxis are negatively regulated by low c-di-GMP levels in P.aeruginosa and thus confront our current undertsandings of c-di-GMP signalling.
HostingRepository	PRIDE
AnnounceDate	2017-09-05
AnnouncementXML	Submission_2017-09-04_23:38:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jessica Rossello
SpeciesList	scientific name: Pseudomonas aeruginosa PAK; NCBI TaxID: 1009714;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-20 01:44:02	ID requested
⏵ 1	2017-09-04 23:38:20	announced

Rossello J, Lima A, Gil M, Rodr, í, guez Duarte J, Correa A, Carvalho PC, Kierbel A, Dur, á, n R, The EAL-domain protein FcsR regulates flagella, chemotaxis and type III secretion system in Pseudomonas aeruginosa by a phosphodiesterase independent mechanism. Sci Rep, 7(1):10281(2017) [pubmed]

curator keyword: Biological

submitter keyword: Pseudomonas aeruginosa

c-di GMP

phosphodiesterase

Rosario Durán
contact affiliation	Lab's head
contact email	duran@pasteur.edu.uy
lab head
Jessica Rossello
contact affiliation	Institut Pasteur
contact email	jrossello@pasteur.edu.uy
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004365
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis of exoproteome and membrane enriched fractions of PAK and PAK pJN2133 strains