PXD004361

PXD004361 is an original dataset announced via ProteomeXchange.

Title	HILIC and ERLIC Enrichment of Glycopeptides Derived from Breast and Brain Cancer Cells
Description	Aberrant glycosylation has been linked to many different cancer types. The blood brain barrier (BBB) is a region of the brain that regulates the entrance of ions, diseases, toxins, etc. However in breast cancer metastasis, the BBB fails to prevent the crossing of the cancer cells into the brain. Here we present a study of identifying and quantifying the glycosylation of six breast and brain cancer cell lines using hydrophilic interaction liquid chromatography (HILIC) and electrostatic repulsion liquid chromatography (ERLIC) enrichments and LC-MS/MS analysis. Qualitative and quantitative analyses of N-linked glycosylation were performed by both enrichment techniques for individual and complementary comparison. Potential cancer glycopeptide biomarkers were identified and confirmed by chemometric and statistical evaluations. The results provide the first comprehensive glycopeptide listing for these six cell lines.
HostingRepository	PRIDE
AnnounceDate	2019-02-27
AnnouncementXML	Submission_2019-02-27_00:22:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jingfu Zhao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-20 01:20:07	ID requested
⏵ 1	2019-02-27 00:22:07	announced

Zacharias LG, Hartmann AK, Song E, Zhao J, Zhu R, Mirzaei P, Mechref Y, HILIC and ERLIC Enrichment of Glycopeptides Derived from Breast and Brain Cancer Cells. J Proteome Res, 15(10):3624-3634(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: glycoproteomics, N-linked glycosylation, HILIC enrichment, ERLIC enrichment, blood brain barrier, cancer cell lines, mass spectrometry

Yehia Mechref
contact affiliation	Department of Chemistry and Biochemistry, Texas Tech University
contact email	yehia.mechref@ttu.edu
lab head
Jingfu Zhao
contact affiliation	Texas Tech University
contact email	jingfu.zhao@ttu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004361
     1. Label: PRIDE project
     2. Name: HILIC and ERLIC Enrichment of Glycopeptides Derived from Breast and Brain Cancer Cells