PXD004351 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Aquifer metaproteomics - Candidate Brocadiales dominates C, N and S cycling in anoxic groundwater of a pristine limestone-fracture aquifer |
Description | Groundwater-derived microorganisms are known to play an important role in biogeochemical C, S and N cycling. Thereby, the presence and majorly the activity of microorganisms in aquifers affect enormously the nutrient cycling. However, the diversity and their functional capability in natural aquifers are still rare and therefore a better knowledge of the core microbial communities is urgently needed. Metaproteome analysis was applied to characterize the repertoire of microbes in the depth and to identify the key drivers of major biogeochemical processes. Therefore, 1000 L water from the aquifer was sampled by filtration on 0.3 µm glass filters. After protein extraction, proteolytic cleavage and mass spectrometric analysis (Ultimate 3000 nanoRSLC coupled to Q Exactive HF instrument), 3808 protein groups (2371 proteins with ≥2 peptides) were identified from 13,204 peptides. The findings of our study have broad implications for the understanding of aquifer cycling’s which finally leads to a greatly improved understanding of the ecosystem services provided by the microbial communities present in aquifers. In the future, functional results would allow to monitor and to assess pollution effects which would beneficially assist groundwater resource management. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:30:33.351.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nico Jehmlich |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-06-16 07:54:53 | ID requested | |
1 | 2016-12-23 10:25:19 | announced | |
2 | 2017-10-24 03:45:45 | announced | Updated project metadata. |
⏵ 3 | 2024-10-22 04:30:36 | announced | 2024-10-22: Updated project metadata. |
Publication List
Starke R, M, ü, ller M, Gaspar M, Marz M, K, ü, sel K, Totsche KU, von Bergen M, Jehmlich N, Candidate Brocadiales dominates C, N and S cycling in anoxic groundwater of a pristine limestone-fracture aquifer. J Proteomics, 152():153-160(2017) [pubmed] |
10.1016/j.jprot.2016.11.003; |
Keyword List
curator keyword: Biological |
submitter keyword: microbial community,groundwater, metaproteomics |
Contact List
Martin von Bergen |
contact affiliation | Helmholtz Centre for Environmental Research - UFZ Head of Department for Molecular Systems Biology http://www.ufz.de/index.php?de=17634 Permoserstr. 15 Building 6, R 521 04318 Leipzig GERMANY Tel.: +49-341-235-1211 Fax: +49-341-235-451211 |
contact email | martin.vonbergen@ufz.de |
lab head | |
Nico Jehmlich |
contact affiliation | Helmholtz-Centre for Environmental Research - UFZ |
contact email | nico.jehmlich@ufz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004351
- Label: PRIDE project
- Name: Aquifer metaproteomics - Candidate Brocadiales dominates C, N and S cycling in anoxic groundwater of a pristine limestone-fracture aquifer