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PXD004351

PXD004351 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAquifer metaproteomics - Candidate Brocadiales dominates C, N and S cycling in anoxic groundwater of a pristine limestone-fracture aquifer
DescriptionGroundwater-derived microorganisms are known to play an important role in biogeochemical C, S and N cycling. Thereby, the presence and majorly the activity of microorganisms in aquifers affect enormously the nutrient cycling. However, the diversity and their functional capability in natural aquifers are still rare and therefore a better knowledge of the core microbial communities is urgently needed. Metaproteome analysis was applied to characterize the repertoire of microbes in the depth and to identify the key drivers of major biogeochemical processes. Therefore, 1000 L water from the aquifer was sampled by filtration on 0.3 µm glass filters. After protein extraction, proteolytic cleavage and mass spectrometric analysis (Ultimate 3000 nanoRSLC coupled to Q Exactive HF instrument), 3808 protein groups (2371 proteins with ≥2 peptides) were identified from 13,204 peptides. The findings of our study have broad implications for the understanding of aquifer cycling’s which finally leads to a greatly improved understanding of the ecosystem services provided by the microbial communities present in aquifers. In the future, functional results would allow to monitor and to assess pollution effects which would beneficially assist groundwater resource management.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:30:33.351.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNico Jehmlich
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-06-16 07:54:53ID requested
12016-12-23 10:25:19announced
22017-10-24 03:45:45announcedUpdated project metadata.
32024-10-22 04:30:36announced2024-10-22: Updated project metadata.
Publication List
Starke R, M, ü, ller M, Gaspar M, Marz M, K, ü, sel K, Totsche KU, von Bergen M, Jehmlich N, Candidate Brocadiales dominates C, N and S cycling in anoxic groundwater of a pristine limestone-fracture aquifer. J Proteomics, 152():153-160(2017) [pubmed]
10.1016/j.jprot.2016.11.003;
Keyword List
curator keyword: Biological
submitter keyword: microbial community,groundwater, metaproteomics
Contact List
Martin von Bergen
contact affiliationHelmholtz Centre for Environmental Research - UFZ Head of Department for Molecular Systems Biology http://www.ufz.de/index.php?de=17634 Permoserstr. 15 Building 6, R 521 04318 Leipzig GERMANY Tel.: +49-341-235-1211 Fax: +49-341-235-451211
contact emailmartin.vonbergen@ufz.de
lab head
Nico Jehmlich
contact affiliationHelmholtz-Centre for Environmental Research - UFZ
contact emailnico.jehmlich@ufz.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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