PXD004343

PXD004343 is an original dataset announced via ProteomeXchange.

Title	Activity-Based Protein Profiling of BRAF Inhibitor Resistance in Melanoma Cell Lines
Description	To investigate mechanisms of resistance to BRAF inhibitor therapy in melanoma, BRAF mutant cell lines have been chronically exposed to BRAFi to create phenotypes with acquired drug resistance. Activity-based protein profiling with desthiobiotinylating ATP probes (ActivX, Thermo) is used to examine the differences between naive and drug-resistant cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:30:20.881.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Koomen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-16 04:05:11	ID requested
1	2016-12-13 14:43:55	announced
2	2017-10-24 02:47:23	announced	Updated project metadata.
⏵ 3	2024-10-22 04:30:21	announced	2024-10-22: Updated project metadata.

Sharma R, Fedorenko I, Spence PT, Sondak VK, Smalley KS, Koomen JM, Activity-Based Protein Profiling Shows Heterogeneous Signaling Adaptations to BRAF Inhibition. J Proteome Res, 15(12):4476-4489(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: ABPP, Kinase Inhibitor,Melanoma, BRAF, Drug Resistance

John Koomen
contact affiliation	Molecular Oncology Chemical Biology and Molecular Medicine Moffitt Cancer Center & Research Institute, SRB3 12902 Magnolia Drive, Tampa, FL, 33612, USA.
contact email	john.koomen@moffitt.org
lab head
John Koomen
contact affiliation	Moffitt Cancer Center
contact email	john.koomen@moffitt.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD004343

PRIDE project URI

• PRIDE
  1. PXD004343
     1. Label: PRIDE project
     2. Name: Activity-Based Protein Profiling of BRAF Inhibitor Resistance in Melanoma Cell Lines