PXD004341

PXD004341 is an original dataset announced via ProteomeXchange.

Title	CHO O-Mannosylation independent of POMT1/2
Description	Protein O-mannosylation is found in yeast and metazoans and a family of conserved orthologous polypeptide O-mannosyltransferases is believed to initiate this important posttranslational modification. We recently discovered that the large families of cadherins and protocadherins carry highly conserved O-Man glycans in specific EC domains, and it was suggested that the function of E-Cadherin was dependent on the O-Man glycans. Deficiencies in the two human polypeptide O-mannosyltransferases, POMT1 and T2, underlie a subgroup of congenital muscular dystrophies (CMD) designated α-dystroglycanopathies, because deficient O-Man glycans on -dystroglycan impair laminin interaction with -dystroglycan and the dystrophin complex. In order to explore the functions of O-Man glycans on cadherins and protocadherins we used a combinatorial gene editing strategy in multiple cell lines to evaluate to the role of the two POMTs initiation O-Man glycosylation and the major enzyme elongating O-Man glycans, the POMGNT1 2GlcNAc-transferase. Surprisingly, we discovered that O-Man glycans on cadherins and protocadherins do not appear to require POMT1 and T2 and moreover that the O-Man glycans on these proteins are not elongated in contrast to those on dystroglycan.
HostingRepository	PRIDE
AnnounceDate	2017-05-18
AnnouncementXML	Submission_2017-05-19_02:43:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adnan Halim
SpeciesList	scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029;
ModificationList	monohydroxylated residue; complex glycosylation; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-16 03:55:59	ID requested
⏵ 1	2017-05-19 02:43:30	announced

Larsen ISB, Narimatsu Y, Joshi HJ, Yang Z, Harrison OJ, Brasch J, Shapiro L, Honig B, Vakhrushev SY, Clausen H, Halim A, -mannosyltransferases 1 and 2. J Biol Chem, 292(27):11586-11598(2017) [pubmed]

curator keyword: Biological

submitter keyword: O-glycosylation, Glycoproteomics, Mannose, O-Mannosyltransferase

Henrik Clausen
contact affiliation	Copenhagen Center for Glycomics and Department of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, DK-2200 Copenhagen N, Denmark
contact email	hclau@sund.ku.dk
lab head
Adnan Halim
contact affiliation	University of Copenhagen
contact email	halim@sund.ku.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004341
     1. Label: PRIDE project
     2. Name: CHO O-Mannosylation independent of POMT1/2