PXD004311

PXD004311 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics of liver from methylmercury-exposed Atlantic cod
Description	In order to investigate the underlying mechanisms of methylmecury (MeHg)-mediated toxicity to Atlantic cod (Gadus morhua), we analyzed the liver proteome of fish exposed in vivo to MeHg (0, 0.5, 2 mg/kg body weight) for 2 weeks. Label-free quantitative mass spectrometry enabled quantification of 1143 proteins, and 125 were differentially regulated between MeHg-treated samples and controls. Six proteins among the top differentially regulated (T23O, GLNA EPS8L2, APOA4, RAP1B, CZTZ) were analyzed using selected reaction monitoring (SRM). Supported by bioinformatics analyses, we conclude that MeHg disrupts mainly redox homeostasis and energy generating metabolic pathways in cod liver, the latter potentially modulated through MeHg-induced oxidative stress.
HostingRepository	PRIDE
AnnounceDate	2016-08-09
AnnouncementXML	Submission_2016-08-09_01:26:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Odd Andre Karlsen
SpeciesList	scientific name: Gadus morhua (Atlantic cod); NCBI TaxID: 8049;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-13 01:46:26	ID requested
⏵ 1	2016-08-09 01:26:06	announced

Yadetie F, Bj, ø, rneklett S, Garberg HK, Oveland E, Berven F, Goks, ø, yr A, Karlsen OA, Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism. BMC Genomics, 17():554(2016) [pubmed]

submitter keyword: Atlantic cod (Gadus morhua), Methylmercury, Liver, Label-free, Orbitrap Velos Pro, SRM, 5500 QTRAP

Odd Andre Karlsen
contact affiliation	Department of Biology, University of Bergen, Bergen, Norway
contact email	Odd.Karlsen@uib.no
lab head
Odd Andre Karlsen
contact affiliation	Department of Biology, University of Bergen, Norway
contact email	odd.karlsen@uib.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004311
     1. Label: PRIDE project
     2. Name: Quantitative proteomics of liver from methylmercury-exposed Atlantic cod