PXD004304

PXD004304 is an original dataset announced via ProteomeXchange.

Title	Analysis of mixed-oxysterol treatment on the THP-1 macrophage
Description	THP-1 monocytes were differentiated, via PMA treatment, to macrophages. THP-1 macrophages were then treated with a mixed-oxysterol treatment (7-ketocholesterol and 7beta-hydroxycholesterol) for 24 hours. The mixed-oxysterol treatment was mixed to a ratio that represents the cytotoxic oxysterol content of human atherosclerotic plaque. Control and treated macrophages were analysed using both 2-DE with peptide mass-fingerprinting and tandem-mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2017-03-15
AnnouncementXML	Submission_2017-04-03_02:14:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liam Ward
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos; Voyager-DE PRO

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-10 02:53:08	ID requested
1	2017-03-15 08:23:54	announced
⏵ 2	2017-04-03 02:14:46	announced	Updated publication reference for PubMed record(s): 28350877.

Ward LJ, Ljunggren SA, Karlsson H, Li W, Yuan XM, Exposure to atheroma-relevant 7-oxysterols causes proteomic alterations in cell death, cellular longevity, and lipid metabolism in THP-1 macrophages. PLoS One, 12(3):e0174475(2017) [pubmed]

curator keyword: Biological

submitter keyword: THP-1, macrophage, oxysterol, proteomics, 2-DE, tandem-mass spectrometry

Xi-Ming Yuan
contact affiliation	Occupational and Environmental Medicine Center, and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
contact email	ximing.yuan@liu.se
lab head
Liam Ward
contact affiliation	Division of Clinical Sciences, Department of Clinical and Experimental Medicine, Linköping University.
contact email	liam.ward@liu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/03/PXD004304

PRIDE project URI

• PRIDE
  1. PXD004304
     1. Label: PRIDE project
     2. Name: Analysis of mixed-oxysterol treatment on the THP-1 macrophage