PXD004303

PXD004303 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Cysteine-targeted proteomics analysis
Description	The protein cysteine residue is one of the amino acids most susceptible to oxidative modifications, frequently caused by oxidative stress. Several applications have enabled cysteine-targeted proteomics analysis with simultaneous detection and quantitation. In this study, we employed a quantitative approach using a set of iodoacetyl-based cysteine reactive isobaric tags (iodoTMT) and evaluated the transient cellular oxidation ratio of free and reversibly modified cysteine thiols under DTT and hydrogen peroxide (H2O2) treatments.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:30:25.115.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kazutaka Araki
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-10 02:15:34	ID requested
1	2016-07-18 00:28:02	announced
⏵ 2	2024-10-22 04:30:25	announced	2024-10-22: Updated project metadata.

Publication List

Araki K, Kusano H, Sasaki N, Tanaka R, Hatta T, Fukui K, Natsume T, Redox Sensitivities of Global Cellular Cysteine Residues under Reductive and Oxidative Stress. J Proteome Res, 15(8):2548-59(2016) [pubmed]

Araki K, Kusano H, Sasaki N, Tanaka R, Hatta T, Fukui K, Natsume T, Redox Sensitivities of Global Cellular Cysteine Residues under Reductive and Oxidative Stress. J Proteome Res, 15(8):2548-59(2016) [pubmed]

Keyword List

curator keyword: Technical, Biological
submitter keyword: HEK293T, iodoTMT6plex

curator keyword: Technical, Biological

submitter keyword: HEK293T, iodoTMT6plex

Contact List

Kazuhiko Fukui
contact affiliation	Molecular Profiling Research Center for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Japan
contact email	k-fukui@aist.go.jp
lab head
Kazutaka Araki
contact affiliation	AIST
contact email	k-araki@aist.go.jp
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD004303
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD004303

PRIDE project URI

Repository Record List

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