PXD004298

PXD004298 is an original dataset announced via ProteomeXchange.

Title	Identifying protein kinase specific effectors of the osmostress response in yeast_part5
Description	Saccharomyces cerevisiae reacts to elevated external osmolarity by several cellular responses. Thereby, the main adaptive signaling activity relies on the high-osmolarity glycerol (HOG) pathway, whose core architecture is closely related to the mammalian p38 MAPK pathway. To identify novel target proteins of its MAP kinase Hog1, we applied an MS-based high throughput analysis measuring the impact of Hog1 activation as well as inhibition on the budding yeast phosphoproteome. In addition, we analyzed how a deletion of RCK2, a known effector protein kinase target of Hog1, modulates the normal stress induced phosphorylation pattern. Our results provide an overview on the diversity of cellular functions that are directly and indirectly affected by the activity of the pathway and allow a clear assessment of Hog1 independent events affected by osmotic stress. Analyzing the modulation of S/T-P motifs, we could extend the number of Hog1 putative direct targets that were subsequently validated by an in vivo interaction assay. It also appears that Rck2, a downstream kinase of Hog1, acts as a central hub for many Hog1-mediated secondary phosphorylation events.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:31:10.315.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wolfgang Reiter
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; 6x(13)C labeled residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-09 05:15:41	ID requested
1	2017-02-01 14:31:00	announced
2	2017-06-12 08:26:35	announced	Updated publication reference for PubMed record(s): 28270554.
⏵ 3	2024-10-22 04:31:11	announced	2024-10-22: Updated project metadata.

10.1126/scisignal.aag2435;

Romanov N, Hollenstein DM, Janschitz M, Ammerer G, Anrather D, Reiter W, Identifying protein kinase-specific effectors of the osmostress response in yeast. Sci Signal, 10(469):(2017) [pubmed]

curator keyword: Biological

submitter keyword: Rck2, Hog1, Yeast, MS shotgun, Saccharomyces cerevisiae,MAPK, Kinase, Phosphorylation

Gustav Ammerer
contact affiliation	Department for Biochemistry, Max F. Perutz Laboratories, University of Vienna, Dr. Bohr-Gasse 9, A-1030
contact email	gustav.ammerer@univie.ac.at
lab head
Wolfgang Reiter
contact affiliation	Mass Spectrometry Facility, Max Perutz Labs Vienna, University of Vienna, Vienna Biocenter, Dr.-Bohr-Gasse 7, 1030 Vienna
contact email	wolfgang.l.reiter@univie.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004298
     1. Label: PRIDE project
     2. Name: Identifying protein kinase specific effectors of the osmostress response in yeast_part5