PXD004295

PXD004295 is an original dataset announced via ProteomeXchange.

Title	Identifying protein kinase specific effectors of the osmostress response in yeast_part2
Description	Saccharomyces cerevisiae reacts to elevated external osmolarity by several cellular responses. Thereby, the main adaptive signaling activity relies on the high-osmolarity glycerol (HOG) pathway, whose core architecture is closely related to the mammalian p38 MAPK pathway. To identify novel target proteins of its MAP kinase Hog1, we applied an MS-based high throughput analysis measuring the impact of Hog1 activation as well as inhibition on the budding yeast phosphoproteome. In addition, we analyzed how a deletion of RCK2, a known effector protein kinase target of Hog1, modulates the normal stress induced phosphorylation pattern. Our results provide an overview on the diversity of cellular functions that are directly and indirectly affected by the activity of the pathway and allow a clear assessment of Hog1 independent events affected by osmotic stress. Analyzing the modulation of S/T-P motifs, we could extend the number of Hog1 putative direct targets that were subsequently validated by an in vivo interaction assay. It also appears that Rck2, a downstream kinase of Hog1, acts as a central hub for many Hog1-mediated secondary phosphorylation events.
HostingRepository	PRIDE
AnnounceDate	2017-02-01
AnnouncementXML	Submission_2017-06-12_08:24:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wolfgang Reiter
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; 6x(13)C labeled residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-09 05:14:29	ID requested
1	2017-02-01 13:27:40	announced
⏵ 2	2017-06-12 08:24:51	announced	Updated publication reference for PubMed record(s): 28270554.

Romanov N, Hollenstein DM, Janschitz M, Ammerer G, Anrather D, Reiter W, Identifying protein kinase-specific effectors of the osmostress response in yeast. Sci Signal, 10(469):(2017) [pubmed]

curator keyword: Biological

submitter keyword: MAPK, Kinase, Phosphorylation, Yeast, MS shotgun, Hog1, Rck2, Saccharomyces cerevisiae

Gustav Ammerer
contact affiliation	Department for Biochemistry, Max F. Perutz Laboratories, University of Vienna, Dr. Bohr-Gasse 9, A-1030
contact email	gustav.ammerer@univie.ac.at
lab head
Wolfgang Reiter
contact affiliation	MAX F. PERUTZ LABORATORIES - University of Vienna
contact email	wolfgang.l.reiter@univie.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/02/PXD004295

PRIDE project URI

• PRIDE
  1. PXD004295
     1. Label: PRIDE project
     2. Name: Identifying protein kinase specific effectors of the osmostress response in yeast_part2