PXD004291

PXD004291 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic analysis of T cell receptor stimulation-induced changes in human primary CD4+CD25- T cells in the absence or presence of regulatory T cells.
Description	Regulatory T cells (Tregs) suppress other immune cells and are indispensable for human health, yet the molecular mechanisms of suppression remain incompletely understood. Tregs can suppress proliferation and cytokine production of CD4+CD25- conventional T cells (Tcons). We previously demonstrated that human Tregs rapidly suppress T cell receptor (TCR)-induced calcium, NFAT and NF-κB pathways in Tcons, leading to inhibition of effector cytokine transcription (Schmidt A et al., Science Signaling 2011 Dec 20;4(204):ra90. doi: 10.1126/scisignal.2002179). Due to the short time period required to induce suppression, it is probable that Tregs alter protein modifications, such as (de)phosphorylations, in suppressed Tcons to cause this inhibition of particular TCR signaling events, while de novo production of repressor proteins seems unlikely. Thus, in order to identify causative factors which initiate rapid Treg-mediated suppression of Tcons, we compared phosphoproteomes of 5 minutes TCR-stimulated responder Tcons re-isolated from cocultures with primary human Tregs with those from control cocultures (Tcons cocultured with other Tcons). In addition, we measured the basal state of the phosphoproteome in unstimulated Tcon of the same donors. In total, we provide phosphoproteomics data of primary human CD4+CD25- T cells from three human healthy donors each for (i) unstimulated Tcons, (ii) 5 minutes TCR-stimulated Tcons, (iii) 5 minutes TCR-stimulated and Treg-suppressed Tcons.
HostingRepository	PRIDE
AnnounceDate	2017-08-28
AnnouncementXML	Submission_2017-10-12_01:42:36.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	N. Binai
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-06-08 07:48:52	ID requested
1	2017-08-28 03:42:42	announced
⏵ 2	2017-10-12 01:42:37	announced	Updated publication reference for PubMed record(s): 28993769.

Joshi RN, Binai NA, Marabita F, Sui Z, Altman A, Heck AJR, Tegn, é, r J, Schmidt A, Phosphoproteomics Reveals Regulatory T Cell-Mediated DEF6 Dephosphorylation That Affects Cytokine Expression in Human Conventional T Cells. Front Immunol, 8():1163(2017) [pubmed]

ProteomeXchange project tag: PRIME-XS Project

curator keyword: Biological, Biomedical

submitter keyword: human CD4+ T cells, co-cultures, regulatory T cells (Tregs), Ti-IMAC, phosphoproteomics

Albert J.R. Heck
contact affiliation	Biomolecular Mass Spectrometry and Proteomics Utrecht University The Netherlands
contact email	a.j.r.heck@uu.nl
lab head
N. Binai
contact affiliation	Faculty of Science
contact email	n.a.binai@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/08/PXD004291

PRIDE project URI

• PRIDE
  1. PXD004291
     1. Label: PRIDE project
     2. Name: Phosphoproteomic analysis of T cell receptor stimulation-induced changes in human primary CD4+CD25- T cells in the absence or presence of regulatory T cells.